体内构建骨软骨复合体修复骨软骨缺损的实验研究(1).docx

文档介绍：体内构建骨软骨复合体修复骨软骨缺损的实验研究(1)
【摘要】 探讨软骨组织工程方法修复骨软骨缺损理想的种子细胞和支架材料。[方法]叭体外诱导骨髓基质干细胞向滁软骨细胞定向分化,分别与秤PLGA支架复合培养,并模仿马赛克骨软骨移植术在屯犬关节骨软骨缺损深层置入顺MSC-支架复合体,浅层荭置入诱导培养后的MSC-支架复合体,紧密压配,体и内构建骨软骨复合体,观察喏其修复的情况。[结果]16周实验组缺损区完全由透盎明软骨修复,与周围正常软低骨完全融合,组织观察显示醚以软骨细胞修复为主,软骨纛下骨形成,潮线基本恢复;糊阴性对照组缺损区主要由纤肠维组织修复,部分由透明软疣骨修复,组织观察显示以纤ゃ维细胞修复为主,混有部分紧软骨细胞;空白对照组完全绿由纤维组织修复。[结论]卓MSCs经向软骨细胞定向分化后复合PLGA支架材媲料,通过紧密压配方式与M糯SCs-PLGA支架在体朗内构建骨软骨复合体,可以葳有效修复骨软骨缺损。编辑适。
【关键词】 骨髓基质治干细胞马赛克移植术骨软骨堕复合体骨软骨缺损
 Ab〕stract:[Obje鼯ctive]Toeval栓uatetheeffec蕊toftreatingt硅heosteochond御raldefectswi烈thimplantedc逡
ellposites,cīulturedMSCsa搁sseedcellsan恣dPLGAasscaff刚olds,andtoac嗜quiredesirab咱leseedcellsandscaffoldma阕terials.[Method]BMSCswereinducedtodi旆fferenciatia憾tedintochond蓟rocytes,coc奚ulturedwithP新LGAscaffoldr〓espectivelyi浍nvitro,theni鹏mplantedinto盟osteochondra狭ldefectsonca醇ninemodelsby砀usingtechniquesofmosaicp桔lasty,induce唬dBMSCpositesinthetop急ofthedefecta法ndBMSCpositesinthebo隽ttomofthedef床ect,osteocho╅posi颍teswereconst筐ructedinvivo杂,andrepairwa箨sobservedwit咬hnakedeyesan枞dhistology.[Result]At16w氪eeksaftertra恸nsplantation绮thedefectsof喙expEirmental榻groupwerecov铂eredwithsemi庚transparent溃smoothwhitet被issueandthem笃arginsbetwee稃ntherepairti细ssueandthesu扈rroundingcar襁tilagewereno,mostofthe蛹repairtissueおwasconsistedwithchondroc〗ytes,maintai赶nedthEIrthic职knesstothefuondralbonewa. Thedefect顽sofpositivec膈ontrolgroupw罪erecoveredwi杪threpairtiss令ues,.However,thedefectsofnega码
tivecontrolg妃roupwererepa塥iredwithsoftfibroustissu钝eswithoutlus恫ter,andanobv摩iousboundary离betweenrepar晔ativeandorig鱼inalcartilag仗ewasseen.[Co揎nclusion]MSC尤sPLGAscaffoposites决,constructedposi鲕tesbysuppres├singcloselyi│nvivo,arethe泷idealmateria骗lsforrepairi朵ngcartilaged珧efects.
Keyw巅ords:bonemes性enchymalstem瞿cells(MSCs);挪mosaicplasty盏;posites吐;osteo