文档介绍：该【第五章信号转导和药物开发 】是由【1781111****】上传分享，文档一共【6】页，该文档可以免费在线阅读，需要了解更多关于【第五章信号转导和药物开发 】的内容，可以使用淘豆网的站内搜索功能，选择自己适合的文档，以下文字是截取该文章内的部分文字，如需要获得完整电子版，请下载此文档到您的设备，方便您编辑和打印。:..信号转导和药物开发因转录因子,后者行使靶基因表达调控功能,引发细胞应答并产生针对信号的生物反应。由此可见,生命科学基础研究中的各种问题,诸如细胞膜结构与功能、蛋白质和蛋白质相互作用、蛋白质和核酸相互作用、蛋白质修饰、基因表达调节控制、细胞周期调节控制、疾病与基因的关系、神经活动的分子机制、内分泌作用及其调节和细胞分化机理等等,在本质上都与不同形式、阶段与内容的信号转导过程有关。因此,研究信号转导,必将有助于阐明这些生物科学基本问题的本质,了解生命奥秘。而且由于细胞中的信号分子和信号诱导基因的产物往往参与多个生物反应,所以各个信号转导过程都不是孤立的;而是通过复杂的相互作用,形成一个个信号网络。信号转导研究在疾病的研究和防治方面还有重要而深远的潜在应用意义。信号转导途径或信号转导分子的异常是造成疾患的分子原因。RasIKi-1Jak/Stat途径异常造成生长激素受体异常症;离子通道途径异常造成遗传性的恶性发热和囊包性纤维病;与GHirschsprung下症候群、慢性髓性白血病;细胞黏附因子异常造成的血小板无力症;细胞周期调节因子异常造成的各种遗传性和后天性癌变,尤其是各种淋巴瘤;基因转录因子异常造成的急性髓性白血病;GP1研究并开发了许多针对信号转导途径的疾病治疗策略和药物,而通过激活或抑制信号转导通如二、磷酸化抗体酶反应转ATPGTPγ基酸残基上的过程,其(ProteinkinasePK)含羟基氨基酸()(ProteinphosphatasePPase)催化磷酸蛋白的酯键水去磷酸化。A4G10?ELISA,FlowCytometry,FluorescencePolarization(FP)Assays,Westernblot(WB),HomogenousTimeResolvedFluorescence(HTRF)Assays,Immuno-cytochemistry(ICC),Immuno-fluorescence(IF),Immuno-histochemistry(IHC),Immuno-precipitation(IP)16-101Anti-Phosphotyrosine(4G10)agaroseconjugate1mg1078816-103Anti-Phosphotyrosine(4G10)biotinconjugate100ug538816-104Anti-Phosphotyrosine(4G10)onjugate100ug538816-105Anti-Phosphotyrosine(4G10)HRPconjugate10blots538805-321Anti-Phosphotyrosine,clone4G10100ug406805-321XAnti-Phosphotyrosine,clone4G1050ug238805-777Anti-Phosphotyrosine,binant4G101mg1738816-184Anti-Phosphotyrosine,binant4G10HRP-Conjugated100ul568816-199Anti-Phosphotyrosine,binant4G10,agaroseconjugate1mg1078816-204Anti-Phosphotyrosine,binant4G10,biotinconjugate100ug538816-205Anti-Phosphotyrosine,binant4G10,FITC-conjugate100ug538817-103Anti-PhosphotyrosineImmunoaffinityPurificationKit1kit1265717-123Anti-PhosphotyrosineImmunoblottingKit(HRP-)1kit700617-153Anti-PhosphotyrosineImmunoblottingKit(4G10),ECL1kit581517-182CellularPhosphotyrosineELISAKit(4G10)1kit623317-315TyrosineKinaseAssayKit,ColorimetricDetection1kit7006:..PlatinumKit4G10PY20效成分,PY204G10和金标准密博通过大量4G10PY20合到外,4A44G10Platinum/4A4样简便(LC/MSMS/MS)05-10504G10Platinum,100uL(includesPC13-302)1kit406805-1051X4G10Platinum,30uL1kit238817-4994G10Platinum/4A4SamplePack1kit1888Ab1603Anti-Phosphoserine50μg276005-1000Anti-Phosphoserine,clone4A4100μg406844-006Anti-Phospho-Serine/Threonine100μg9386Phospho-Threonine(42H4)MousemAb100μl243817-269Phospho-Serine/ThreonineAssayKit,Chemiluminescence96well53972、MAPKMARKERK1/2JNK/SAPKp38MAPKERK1/2裂号进行传导,而JNK/SAPp38MAPK类型的细胞应激信号进当然传导一种或一种以上细胞外信号。信号转导途径间还存crosstalkMAPK细胞增殖与分化、细胞凋亡领域A、ERK1/2生长//ERK1/2号通过生长因子受体和酪氨酸激酶介Ras,c-Raf(Ser259/Ser621)MEK1/2(Ser217/221)ERK1/2(Thr202/Tyr204:..人激ERK1/2Elk-1(Ser383)Elk-1SREERK1/2Stat3(Ser727)c-Myc(Thr58/Ser62)c-Myc结合E-BoxERK1/2p90RSK(Ser381)见下页列表。、p38MAPK细胞应激,包括渗透压多糖(LPS)p38MAPK炎性细胞因子依次MEKK1SEK1/MKK4/JNKKp38MAPK(Thr180/Tyr182)MKK3(Ser189/Thr193)/MKK6(Ser207/Thr211)也是p38MAPKp38MAPKMAPKAPkinase-2p38MAPKElk-1ATF-2MaxCREBAllenM2000p38alphaCSAPK/JNKSAPK/JNK性MEKK1,SEK1/MKK4/JNKK(Ser219/Thr223)SAPK/JNK(Thr183/Tyr185)SAPK/JNKc-Jun(Ser63/Ser73)c-JunTRE/AP-1SAPK/JNKATF-2/CRE-BP1(Thr69/Thr71),ATF-2AP-1CREDNASAPK/JNK可激活转录因子Elk12、Akt作/Akt/PKB增根据产生的组织特异性不同,将Akt/PKBAkt1/PKBaAkt2/PKBbAkt3/PKBrAkt3/PKBr则有严格Akt/PKB480NPH区和CPH1003-磷脂酰PH结构域有高度的相似性,故PHAkt/PKB与3-Akt/PKB分子质膜移位从被活化。激酶活性区位于中部,PKAPKCAkt/PKBAtk1/PKBaT308位点)亲水CAkt1/PKBaS473T308S473的磷酸化是活化Akt/PKB[]MAPKAKT体产9271SPhospho-Akt(Ser473)Antibody100ul32759211SPhospho-p38MAPKinase(Thr180/Tyr182)Antibody200ul32759101SPhospho-p44/42MAPKinase(Thr202/Tyr204)Antibody200ul32759272AktAntibody100ul25509251SPhospho-SAPK/JNK(Thr183/Tyr185)Antibody200ul32759212p38MAPKinaseAntibody200ul25509106SPhospho-p44/42MAPK(Thr202/Tyr204)(E10)MousemAb200ul26759102p44/42MAPKinaseAntibody200ul25509216SPhospho-p38MAPK(Thr180/Tyr182)(28B10)MousemAb100ul26759252SAPK/JNKAntibody200ul25509255SPhospho-SAPK/JNK(Thr183/Tyr185)(G9)MousemAb200ul26759242IkappaB-alphaAntibody100ul25509542PARPAntibody100ul25503787SPhospho-Akt(Ser473)(736E11)RabbitmAb200ul32754051SPhospho-Akt(Ser473)(587F11)MousemAb100ul2675Phospho-p44/42MAPK(Thr202/Tyr204)(20G11)RabbitmAb(IHC4376S200ul3275Preferred)9101SPhospho-p44/42MAPKinase(Thr202/Tyr204)Antibody200ul32759131SPhospho-Stat3(Tyr705)Antibody100ul32759131LPhospho-Stat3(Tyr705)Antibody300ul78629171SPhospho-Stat1(Tyr701)Antibody100ul32759191SPhospho-CREB(Ser133)Antibody100ul3275:..Phospho-IkappaB-alpha(Ser32/36)(5A5)MousemAb100ul26759271LPhospho-Akt(Ser473)Antibody300ul78629275SPhospho-Akt(Thr308)Antibody100ul32752101SPhospho-SrcFamily(Tyr416)Antibody100ul32752531SPhospho-AMPKalpha(Thr172)Antibody100ul32752535SPhospho-AMPKalpha(Thr172)(40H9)RabbitmAb100ul32759336SPhospho-GSK-3beta(Ser9)Antibody100ul32754058SPhospho-Akt(Ser473)(193H12)RabbitmAb100ul32754058LPhospho-Akt(Ser473)(193H12)RabbitmAb300ul786244740GPhospho-Tau(Thr212)RabbitpAb100ul420044750GPhospho-Tau(Ser262)RabbitpAb100ul420044764GPhospho-Tau(Ser422)RabbitpAb100ul4200、AntibodySamplerKit这是一种包括多种的经济实惠合,samplerKit足4westernmin-blot能帮您快速、经的成科研进设计的指标或课题包括AMPK&ACC,蛋白,H3,Akt,钙粘-连环素,细胞周期调控,组蛋白去乙酰(HDAC)JakMAPKNF-kappaBAkt,酸化Chk1/2磷EGFestrogen受体alphaHER2/ErbB2磷MAPK酸化PKCSAPK/JNKStatSykp53,前期凋亡Bcl-2Raf家族SrcStatCaspase剪切Erk1/2酸化p38MAPK以下是部分产品,更多产与我们联系:9910Phospho-MAPKFamilyAntibodySamplerKit3x40ul28759916Phospho-AktPathwaySamplerKit7x40ul49759926MAPKFamilyAntibodySamplerKit3x40ul28759932CellCycleRegulationSamplerKit8x40ul5500三、磷酸化蛋白ELISA-phosphoELISA?phosphoELISA?了一种简单但功能的新法,ELISA技术96孔板中高通量检测,简单高效;定量的结果能更深研究信号动态变化。phosphoELISA?步骤:夹心法ELISA96的;检测抗体是磷酸化特异性的(如Akt[pS473]物phosphoELISA?可以用来进行:??多细胞研究?不同激下体内或体外应答?蛋白活性及功能调节分子研究?WesternblottingRNAi、[产品信息]以下产Akt为例更多ELISA与KHO0201Akt[pT308]phosphoELISA?,968320KHO0111Akt[pS473]phosphoELISA?,与人、小鼠、大鼠反应96孔8320KHO0101Akt(total)phosphoELISA?,96孔8320四、激酶及检测试剂盒:..、激酶及其底物激酶通过酸化作用在细胞信号转导中起到重要而广泛的作用,人类酶活性已经成为一种疾病治疗策略,30%高通路路研究、激酶抑制剂或激活剂开发等必需的生物分子。以下激酶产品为常用种类,多产我们联系:14-276Akt1/PKBalpha,active15ug406814-305AMPK,active5units406814-346CHK1,active10ug406814-347CHK2,active10ug406814-450cdk1/cyclinb,active10ug406814-451ROKalpha/ROCK-II,active10ug406814-475cdk2/cyclinE,active10ug406814-476cdk7/cyclinh/mat1,active10ug406814-525PKCζ,active10ug40687394AuroraBKinase5ug243812-373CHKtide(CHK1/CHK2substratepeptide)1mg214812-411PKCSubstratePeptideII1mg214812-439Rb(aa773-928)500ug23282、激酶检测试剂盒激酶检测试剂盒(非放射性)AktKinaseAssayKit(Nonradioactive)#9840--Western准确激酶活性:免疫固Akt(1G1)抗体从细胞中将AktGSK-3合蛋白进Phospho-GSK-3alpha/beta(Ser21/9)WB检GSK-3而Akt性。非放射性的AktAkt,甚至Western所需及发光PDGFNIH/3T3细胞检测AKT以下激酶检测试剂盒为常用种类,更多产品请与我们联系:9840AktKinaseAssayKit40assays43759820P38MAPKinaseAssayKit40assays4375五、抑制剂&抑制剂、拮抗剂是信号转导研究中的重要工具,我们提供以下激酶或通的抑制Akt,CellCycle,GTPase,GSK-3,Jak/Stat,LipidKinase,MapKinase,PKA,PKC,RafKinase,RhoKinase,Ribosomal,Serine/ThreoninePhosphatase,Serine/ThreonineProteinKinase,TyrosineKinase…TargetPHZ1173AG1024TyrKinase1mg960PHZ1204AG490TyrKinase5mg620PHZ1083GF109203XSer/ThrKinase1mg1340PHZ1094Go6976Ser/:..PD153035TyrKinase1mg1220PHZ1133PD169316MapKinase1mg1220PHZ1164PD98059Ser/ThrKinase5mg1100PHZ1233RapamycinSer/ThrKinase1mg2080PHZ1253SB203580Ser/、染色质研究近年组蛋白乙酰化和去乙酰化是调节基因表达的关键环节之一,EMSA相,基于体内分析而发展的染色质免疫沉淀分析Chromatinimmunoprecipitationassay,ChIP)整DNA序列上的调控蛋白,是定与确法。这一早期曾多用于研究核小体上的DNA近年DNA可用于高通量的筛选已知蛋白因子的未知DNA个基因情况,DNA-蛋白质相互作1、234、有丝分裂研究、5DNA损伤分具体介及产品信息请参考第四章第三节“观遗传学”染色质改型(ChIP染色质免疫沉淀)