文档介绍：临床医学论文内***酸乙酯对D-氨基半乳糖诱导的急性肝损伤大鼠的保护作用【摘要】目的观察丙删酸乙酯(EP)对D-氨基半乳糖盐酸盐诱导的急性肝损伤模羽人鼠的保护作用,并探讨其作用机制。方法制备D-氨基半乳糖盐酸盐诱导的急性肝损伤人鼠模型48只,并随机分成正常组、模型组、小剂量EP提前干预组、人剂虽EP提前干预组、小剂量EP治疗组和人剂量EP治疗组。在造模后24h时取血,采用ELISA法检测TNF-a>IFN-Y、IL-10和IL-18水平;采用RT-PCR法检测肝组织HMGB1mRNA水平变化。结果与正常组比较,模型组大鼠血清TNF-a、IFN-y、1L-10和IL-18水平均无明显变化(),而肝组织HMGB1mRNA水平明显升高(P<);与模型组比较,EP提前干预组和EP治疗组动物血清TNF-a、TFN-y>TL-10和TL-18水平均无明显变化,而EP提前干预则显著降低了肝组织HMGB1mRNA水平(P〈),其中人剂量组绘为显护(P<):病理学检杳显示,EP提前下预组大鼠肝组织学得到明显改善,尤其是在大剂量组,而H比EP治疗组更加显苦。结论EP能有效保护D-氨基半乳糖盐酸盐诱导的大鼠急性肝损伤,保护效果与药物的干预剂量成正相关,并且捉前干预的效果更明显。【关键词】急性肝损伤;丙涮酸乙酯;高迁移率族蛋白Bl;D-氨基半乳糖TheprotectiveeffectofethylpyruvateonacutehepaticinjuryinducedbyD-galactostiminehydrochlorateinratsHECanming,WANGLuwen,RENQiaojing,etal・DepartmentofInfectiousDisease,People"sHospital,WuhanUniversity,Wuhan,430060>China【Abstract]ObjectiveToobservetheprotectiveeffectofethylpyruvate(EP)onacutehepaticinjuryinducedbyD-galactosaminehydrochlorateinras・MethodsTheacutehepaticinjuryinducedbyD-galactostiminehydrochloratewerepreparedin48maleWistarratsandtheanimalsweredividedatrandomintononnal,model,earlyinterfereneeoflowdoseofEP,earlyinterfereneeofhighdoseofEP,lowdoseofEPtreatmentandhighdoseofEPtreatmentgroups・24hoursaftermodeling,thescrumlevelsofTNF-a,IFN-Y,1L-10and1L-18wereassayedbyELISA,thechangesofhighmobilitygroupbox1protein(I1MGB1)mRNAinlivertissuesweredeterminedbyRT-PCR,(P<),whileserumlevelsofTNF-a,TFN-Y»TL-10andTL-18inmodelgrouphadnoobviouschanges(P>)・Comparedwithmodelgroup,thelevelsofserumTNF-a,IFN-Y,IL-10andIL-18hadnoobviouschangesinEPearlyinterferencegrouporinEPtreatmentgroup,butthe11MGB1mRNAlevelsinlivertissuesinEPearlyinterfereneegroupwereobviouslydecreased(P〈0・01),especiallyinhigh-dosegroup,whichwasmoreobviousthaninEPtreatmentgroup(P<)・ThehistopathologicalchangesintheliverswasmostsignificantlyimprovedinEPearlyinterferenee,especi