文档介绍：广 东 药 科 大 学 学 报
Journal of Guangdong Pharmaceutical University Jul. 2021，37（4）
esearch and Development in Traditional Chinese Medicine,
Guangzhou 510095, China; 3. School of the Fifth Clinical Medicine, Guangzhou University of Chinese Medicine,
Guangzhou 510405, China)
*Corresponding author Email: ******@
ABSTRACT: Objective To establish the UPLC fingerprint of Yinlan Hanshufang and simultaneous
determination method of 6 components, so as to provide a reference for the quality control of Yinlan
Hanshufang. Methods The column was Phenomenex Luna Omega PS column C ( mm×150 mm, μm)
18
with a mobile phase of acetonitrile-% formic acid (% phosphoric acid) in gradient elution. The flow rate
was mL/min, the column temperature was 30 ° C, and the detection wavelength was set at 245 nm. The
quality of 15 batches of Yinlan Hanshufang was evaluated by similarity analysis, PCA and PLS-DA of
fingerprints, and the content of 6 components, including glycyrrhizin, baicalin, wogonoside, baicalein,
glycyrrhizic acid and wogonin was determined simultaneously. Results The UPLC fingerprint of Yinlan
Hanshufang was established, and thirteen peaks were selected as the character fingerprint pea