文档介绍：: .
中国无机分析
胺作为荧光探针，基于其荧光猝灭程度与 Hg(Ⅱ)浓度呈线性关系，建立了荧光光度法测定水质样品中溶解
态汞的检测方法。考察了缓冲体系的类型、pH 值、浓度以及荧光探针的浓度等条件，确定采用 pH .0 =5 的
aAcHAc-N 缓冲溶液( 0100. mol/L )体系和 μg/mL 胺荧光探针的荧光探针实验条件，并应用于实的对苯二
际水质样品中。结果表明，当 Hg(Ⅱ)浓度在 ~ μg/mL，荧光探针对苯二胺的荧光淬灭程度与 Hg(Ⅱ)
浓度成良好的线性关系( = )，重复性实验的相对标准偏差为 %，方法检出限为 mL μg/ ，加
标回收率均在 %~105%。方法简便、灵敏度高、选择性好、可用于实际水质样品中溶解态汞的测定，
具有较好的应用前景。
关键词 荧光探针；溶解态汞；对苯二胺；荧光猝灭；荧光光度法
Determination Mercury in Water Samples by
P-phenylenediamine as Fluorescent Probe
YU Yuanyuan1, XIAO Jie1, FANG Chao1,2, LI Lingfeng1, LIU Ling1,2,
HAOZ Hai 1,LI Jianchao1, SONG Hongyuan1
( 1． Sixth Geological Brigade of Hubei Geological Bureau，Xiaogan, Hubei 432000, China;
2． Hubei Chupeng Testing Science & Technology Co. Ltd.，Xiaogan, Hubei 432000, China)
Abstract A detection system of dissolved mercury based on p-phenylenediamine fluorescent probe was
established by fluorescence spectrophotometry to simply and sensitively etect d the dissolved mercury in water
samples, The fluorescence probe used p-phenylenediamine as the fluorescence group and its amino group as the
active group. Based on t