文档介绍:分子植物育种
Molecular Plant Breeding
ISSN 1672-416X,CN 46-1068/S
性或者碱性,大部分家族成员定位在细胞膜;33 个成员中鉴定到了 10 个 motif,
通过构建系统进化树可以将 ALA 家族成员分为 5 个簇;顺式作用元件分析发现 ZmALA 家族成员含有光
响应、缺氧、低温、激素响应元件;通过转录组数据分析发现大部分 ZmALA 家族成员在热胁迫下的叶片
中表达量发生显著变化,实时荧光定量 PCR 分析验证了这些基因参与了植物热胁迫响应。本研究结果为后
续开展玉米 ALA 基因在热胁迫响应中的研究提供了科学依据。
关键词 玉米; ALA 基因家族; 生物信息学; 基因表达; 热胁迫
Genome-wide Analysis of the ALA Gene Family in Maize
Wu Fei* Qin Lian* Guo Yiting Ye Juan Huang Min Li Manfei Du Hewei** Liu Rui**
Yangtze University of Life Sciences, Jingzhou, 434000
* These authors contributed equally to this work
** Co-corresponding authors, ******@; liuxiaoshuang_******@
Abstract To investigate the function of Aminophospholipid ATPase (ALA) gene in maize (Zea mays) growth
and development under abiotic stress and stress response, members of the maize ALAgene family were identified
by bioinformatics approach, the chromosomal distribution, protein physiochemical properties, phylogenetic
evolution, cis-acting elements in promoters and expression patterns were analyzed. Results showed that there are
33 ZmALA genes