银杏愈伤组织培养及银杏内酯测定的研究_英文_.pdf

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第 1 6 卷第 1 期沈阳药科大学学报 Vol 16 No 1
1 9 9 9 年 1 月 Journal of Shenyang Pharmaceutical University Jan 1999 p 10
银杏愈伤组织培养及银杏内酯测定的研究
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郑玉果于荣敏1 姚新生张辉邵刚
沈阳药科大学中药系,沈阳 110015
摘要国内首次采用固体培养的方法对组织培养法生产银杏内酯进行了研究. 考察了银杏不同
外植体的愈伤组织的诱导情况;对得到的各种愈伤组织进行了继代驯化,并考察了光照、MS 培养
基成分等多种理化因子及激素对愈伤组织的诱导及生长的影响;应用生物法( PAF) 和 HPLC 对愈
伤组织中的银杏内酯 A 和 B 进行了测试. 结果显示,愈伤组织中的银杏内酯 A 和 B 的含量在 10 ×
10 - 6~50 × 10 - 6之间.
关键词银杏;愈伤组织培养;银杏内脂; HPLC
分类号 Q946
1 Introduction
Ginkgo biloba tree is the only living species of the order Ginkgoales ,whose ancestry has been
traced to the J urassic period. Baiguo ,Semen Ginkgo is the dry ripe seeds of the plant collected in
the fall when the seeds are ripe. This crude drug is officially listed in the Chinese Pharmacopoeia
and used in a traditional Chinese medicine as an antiasthmatic and against polyuria.
Ginkgolides ( Fig. 1) are obtained from the root , bark ,and leaves of the G. biloba tree〔1〕.
Ginkgolides are specific platelet aggregating factor ( PAF) antagonists. PAF is a potent mediator of
inflammation ,which is secreted by a variety of leucocytes and serves to increase vascular perme
ability and elicit chemotaxis and aggregation.
PAF has been implicated as a mediator in a num
ber of clinical conditions ranging from asthma
and various allergic disorders to transplant rejec
tion , shock states , such as sept