文档介绍：化瘀通脉颗粒质量标准研究
胡靖1 吴勇2
(1马鞍山十七冶医院安徽马鞍山 243000; 2马鞍山市食品药品检验所安徽马鞍山 243000)
【摘要】目的建立化瘀通脉颗粒的质量标准。方法采用薄层色谱法对化瘀通脉颗粒中的丹参、白芍、当归、川芎进行了定性鉴别;用HPLC测定制剂中芍药苷的含量,采用Kromasil C18色谱柱;乙腈-水(15:85)为流动相;检测波长为230 nm; ml•min-1。结果定性鉴别方法专属性强;~μg•ml-1范围内有良好线性关系,回归方程为Y = 11 946 X + ,R= 9(n=6),%,(n=4)。结论本研究建立的TLC和HPLC方法专属性强、灵敏度高、重现性好,可用于化瘀通脉颗粒的质量控制。
【关键词】化瘀通脉颗粒质量标准薄层色谱法液相色谱法芍药苷
【中图分类号】 【文献标识码】A 【文章编号】2095-1752(2012)14-0377-03
Study on the Quality Standard of Huayu Tongmai Granules
HU jing1 WU yong2
(Maanshan Hospital of 17th Metallurgical pang. Maanshan AnHui 243000, Maanshan institute for food and drug control, Maanshan AnHui 243000)
【Abstract】 Objective:To establish quality standard of Huayu Tongmai :TLC method was used to identify Danshen, Baishao, Danggui, Chuanxiong, HPLC method was used to determinate paeonilorin in this preparation. The separation was preformed on Kromasil C18column, using Acetonitrile-water(15:85), the detecting wave length was 230nm and flow rate was •min-:Qualitative identification method has high specificity, the
linear range of Paeonilorin was ~ μ -1, recursive equation was Y = 11 946 X + ,R= 9(n=6), the average recovery was %, RSD=%(n=4).Conclusion:The quality standard based on TLC and HPLC method has high specificity, high s