保肝解毒颗粒对雷公藤多苷致急性肝损伤小鼠自由基脂质过氧化反应的影响.doc

文档介绍：保肝解毒颗粒对雷公藤多苷致急性肝损伤小鼠自由基脂质过氧化反应的影响
【关键词】肝脏疾病中医药疗法动物模雷公藤多苷脂质过氧化保肝解毒颗粒
ABSTRACT: Objective To explore the effects of Baogan Jiedu Granule on lipid peroxidation of free radicals in acute liver injury mice induced by tripterygium glycosides. Methods Totally 60 mice ly divided into tripterygium glycosides model group, largedose, mediumdose and smalldose groups of Baogan Jiedu Granule, Ganlixin group and control group. Each group glycosides ake the model. The serum levels of ALT, AST, SOD, MDA and GSHPx easured. Results The serum levels of SOD and GSHPx in model group decreased and MDA rose obviously. pared odel group, Baogan Jiedu Granule of all doses could effectively decrease ALT, AST and MDA level and obviously improve GSHPx level in each group. In addition, in the largedose group SOD level arkedly improved (P<). Conclusion Baogan Jiedu Granule has obvious therapeutic effects on liver injury through decreasing serum transaminase level and antilipid peroxidation of free radicals.
KEY DA)、谷胱甘肽过氧化物酶(GSHPx)的影响,进一步阐明了DILI的发病机理及保肝解毒颗粒的作用机制。
1 材料与方法
实验动物昆明种小鼠,体重18-22g,雌雄各半,购自河南省实验动物中心(许可证号:410115)。以普通鼠料喂养于河南中医学院动物实验中心,鼠料购自河南省实验动物中心(许可证号:4104041)。
药物与试剂保肝解毒颗粒由芦荟、刺蒺藜、大枣等组成,(),由河南中医学院第一附属医院制剂室制备(批号20041220);雷公藤多苷片,上海复旦复华药业有限公司生产(批号040303);甘利欣胶囊由江苏正大天晴药业股份有限公司生产(批号040801);ALT试剂盒(批号040707)、AST试剂盒(批号040707)、SOD试剂盒(批号20040114)、MDA试剂盒(批号200501