文档介绍：422 中华艰底病杂志年月第卷第痗 Jol隢.】. 6
·论著·
缺氧诱导因子一和血管内皮生长因子干扰性
RNA
生长因子表达的抑制
蒋剑夏晓波许惠卓熊思齐刘双珍李岩
【摘要】目的探讨缺氧诱导因子一一脱9苣谄どひ蜃∑胃扇判訰
(sIRNA)VEGF方法 HIF1nsiRNA
(HuVEc12)(20)(1)Lipofectamine
。直鹱H究赵靥逯柿空载体组一、皊
和猭。 s|RNA()
反应狿ɑ蜃H拘蕔 PcRvEGF
的变化。结果 H1Fh stRNA24螅琀一 siRNA
VEGFsiRNAVEGF和蛋白表达,而低氧组表达明
HIFlaVEGF结论
蚔鑣能有效抑制人血管内皮细胞的表达。
【关键词】缺氧诱导因子琣亚基;血管内皮生长因子类‘扇舽内皮细胞
中围分类号: .
帅 elpressioof誧 endotheJial interferIng RNA targeting hypoxia
inducibIe — and 阹躷 buman endotheaJcesJAG
JitxlA xtt 騴,斟 D4mnf0phthimolgXinngynHspitCr4z
“抽眎,Ⅱ”蕖蓿珻
co5dingdf^0rX^xmoEmzl@^co2
【】 objective investlgate the small 癵 hypoxia
inducible factor1(HIF vascularendotheIlal growh
human endothelial ceIls Methods blnant Dlasmld was constructed
Human ellal cls vltro and divided %
hypoxl8 group(102)Hypoxla970up was thenvlded controI 瑅。瓾一 group
一, groupVEFl siRNA)and cotransfectiogroup(H1Fh3lRNA+VEGFl
slRNA)M∞ pIasmid was transfected ceIls group
the control gropThe HlF1siRNAand VEGFl65 slRNA bInant plasmId
reverse transcrlptasePoIymerasechain 狿甌
and protein RTPcRand . Res The
H1F slRNA and VEGFm sIRNA binant 縜 hours transfectdThe
and protein was faint 騮産 increased obviousIy
hypoxl8 甌 and protein the HlF 1VEGFand —
lower , inecontroI groupCo癵 the highest inKbitory
. Ⅱ HIF1nand VE(F165slRNA can effectively inhibit the human
endothelial cells
【 words Hypoxia inducibIe 琣 subun|t endot