文档介绍：Product Contents
Alkaline Phosphatase, Calf Intestinal (CIAP): Part# 9PIM182
Part No. Conc. (u/µl) Size (units)
M182A 1 1,000 Revised 1/07
M282A 20 1,000
Description: Alkaline Phosphatase catalyzes the hydrolysis of 5´-phosphate groups from DNA, RNA and both ribo- and
deoxyribonucleoside triphosphates.
Enzyme Storage Buffer: Alkaline Phosphatase, Calf Intestinal (CIAP), is supplied in 10mM Tris-HCl (pH ), 1mM MgCl2,
ZnCl2, 50mM KCl and 50% glycerol.
Alkaline Phosphatase 10X Reaction Buffer (M183A): When the 10X Reaction Buffer supplied with this enzyme is diluted
1:10, it has position of 50mM Tris-HCl (pH at 25°C), 1mM MgCl2, ZnCl2 and 1mM spermidine. AF9PI M182 0107M182
Source: Calf intestinal mucosa.
Unit Definition: One unit is defined as the amount of enzyme required to catalyze the hydrolysis of 1µmol of 4-nitrophenyl
phosphate per minute at 37°C in 1M diethanolamine, 4-nitrophenyl phosphate, MgCl2 (pH ). See the unit
concentration on the Product Information Label.
Storage Temperature: For long-term storage (infrequent use; 1–2 times per month), store at –70°C. For daily/weekly use,
store at –20°C. Avoid multiple freeze-thaw cycles. See the expiration date on the Product Information Label.
Quality Control Assays
Contaminant Activity
Endonuclease Assay: To test for endonuclease activity, 1µg of Type I supercoiled plas