文档介绍：中国农学通报 2009,25(20):54-57
Chinese Agricultural Science Bulletin
小麦醇溶蛋白酸性聚丙烯酰胺凝胶电泳
方法的优化及应用
王祖华,杨瑞先,姬云波
(洛阳理工学院环境与化学系,河南洛阳 471023)
摘要:为了形成一套适合实际情况的小麦醇溶蛋白酸性聚丙烯酰胺凝胶电泳(A-PAGE)方法和谱带分
析技术;依据国际种子检验协会1986年公布的标准小麦醇溶蛋白A-PAGE方法,结合国内实验室的实际
情况,摸索出了适合中国试剂和仪器的小麦醇溶蛋白 A-PAGE 的改良方法。该方法克服了标准方法中
的胶韧性差、剥胶困难、分辨率不高、谱带识别繁琐、实验效率较低的限制。该方法适合中国小麦品种纯
度的快速鉴定、血缘关系分析、遗传育种种质材料的选择等。
关键词:小麦;醇溶蛋白;A-PAGE;改良;韧性
中图分类号: 文献标识码:A 论文编号:2009-1102
Improvement and Its Application of Acid Polyacrylamide
Gel Electrophoresis of Wheat Gliadin
Wang Zuhua, Yang Ruixian, Ji Yunbo
( , Luoyang 471023)
Environment and Chemical Department, Luoyang Polytechnic
This paper is to formulate a set of acid polyacrylamide gel electrophoresis of wheat gliadin and the
Abstract:
analytical technique of the bands suiting our actual conditions. Based on the standard acid polyacrylamide gel
electrophoresis of wheat gliadin was published by ISTA in 1986, combined with the actual circumstance of na⁃
tional laboratories, and explore the improved methods of the acid polyacrylamide gel electrophoresis of wheat
gliadin of the reagents and equipment for China. This method overcame the many restrictions of the standard
method, such as the poor gel toughness, the gel desquamated fortably, low resolution, troublesome identi⁃
fication of the bands, less efficiency of the experiment. This method suited the