文档介绍：油脂开发粮油食品科技第 13卷2005年第1期
大豆胚芽中提纯皂甙和异黄酮的研究
贾建波,李东
(淮阴工学院生物工程系,江荪淮阴 223001)
摘要:探讨了以水为介质,对大豆胚芽中的皂甙和异黄酮进行微波和超声波前处理,提取效果
和纯溶剂萃取法相比,经正交实验,结果表明:经450W功率微波处理 30min,400W功率超声波在
40℃处理 45min,固液比为 1:20,温度为 60℃,时间为 1h,再经 40%的乙醇,浸提 2次。异黄酮提取率
从 %提高到 %,皂甙从 %提高到 %,比溶剂法的提取率分别提高了 43%和 32%,经
聚酰胺柱层析分离纯化,大豆异黄酮和大豆皂甙产品纯度达到 38%和 94%。
关键词:微波和超声波;大豆胚芽;大豆异黄酮;大豆皂甙
中图分类号: 文献标识码:A 文章编号:1007—7561(2005)01—40—02
Studies onpurification of isoflavone and soysaponin from
soybean germ by microwave and ultrasound
JIA Jian—bo,LI Dong
(Huai Yin Institute of Technology,Huai of Yin(Huai YinInstitute 223001)
Abstract:Isoflavone and soysaponin from the soybean germ was predisposed in the water by mlcmwave and u1.
effeciency whichof Was contrasted with solvent orthogonal resultin~
ed that the material disposedafter with 450W microwave for30min and 400W ultrawave45min for at
ratio ratio solidof and liquid Was 1:20,then extracted by40% ethanol twice 60~Cat isoflavone recovery
Was increased from % to % ,the soysaponin Was increased from %% to ,and were higherthan
solvent extraction 43% extraction solvent and32% Was38% andsoysaponin isoflavone of and94% by
polyamide column.
Key words:microwave and ultrasound;soybean germ;isoflavone;soysaponin
大豆胚芽是大豆加 T 业中的副产