文档介绍：Study on the chemical consitituents from Onychium lucidum and the Pharmacological activity of Onychin
Abstract
Objective: Take the study on the chemical consitituents from Onychium lucidum and the pharmacological activity of onychin. To provide basis for obtaining anti-cancer and anti-oxidation pounds with strong biological activity and low toxicity.
Methods: (1) Isolate and analyze the effective constituents of Onychium lucidum by using modern separation technology. Then we identified their structures by the means of many anic spectral analysis, such as IR, MS, 1H-NMR, 13C-NMR etc.
(2) The anti-cancer activity of onychin on MGC-803 cell lines, HeLa cell lines and HepG2-A16 cell lines was evaluated by the MTT-based assay. The anti-cancer effect of onychin on the survival time of mice inoculated with Ehrlich ascites carcinoma was detected in vivo. (3) Study the anti-oxidant activity and the mechanism of onychin on oxidative damage of vascular endothelial cells by animal experimental method and MTT-based assay. (4) Take the acute toxicity study of onychin on normal SD rat models by using animal experimental method.
Results: (1) pounds were isolated from Onychium lucidum, and thirteen of their structures were identified. (2) Onychin had anti-proliferaive effect and anti-colony forming ability on MGC-803 cell lines, HeLa cell lines and HepG2-A16 cell lines. Onychin could extended the survival time of mice inoculated with Ehrlich ascites carcinoma. (3) The results of onychin’s in vitro antioxidant activity test showed that onychin had a protective effect on vascular endothelial function which was damaged by oxidants. (4) The rats were given intraperitoneal injection of Onychin of 30mg·kg-1 and 150mg·kg-1 each day for 7 days, no rats showed toxicity change in the appearance, hemogram, liver and kidney function, a variety ans,
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tissues and bone marrow.
Conclusion: (1) pounds were isolated from Onychium lucidum, and thirteen of their structures were identified; (2)