文档介绍：CEA实时荧光定量RT论文
葛明建,时德吴庆琛,王梅,李强,李良彬
【关键词】胞肺癌患
【Abstract】 AIM: To assess anoeuvre or resection of lung cancer could lead to haematogenous dissemination of malignant cells, to monito the quantity and timing of the shedding of lung cancer cells into the circulation of patients by fqRTPCR before, during and after surgery, and to determine the relationship betatogenous dissemination of cancer cells during operation. METHODS: Sixtynine peripheral blood samples 23 consecutive patients all cell lung cancer ly assigned before the operation to 2 surgical procedure groups according to the order of vessel ligation (PVfirst group and PAfirst group). Additionally, 10 patients ples bryonic antigen as marker. RESULTS: The level of CEA mRNA in peripheral blood (PB) ascended continuously a and squamous carcinoma (P=). The values of CEA mRNA in PB bete time, there RNA in PB (P=). Among the 23 cases, 10 cases all cell lung cancer might be regarded as having systemic disease, our staging method. CEAexpressing tumour cells are disseminated mostly postoperatively,.freelay potentially be the source of recurrence or metastases. Surgical manipulation can promote the release of tumour cells into the blood stream, but the ligation of PV before the ligation of the PA may partly prevent such release during surgery.
【Key; blood; polymerase chain reaction; carcinoembryonic antigen; messenger R