文档介绍：楮实提取物体外抗氧化活性的研究
作者:吴兰芳张振东景永帅杨娟
【摘要】目的探讨楮实提取物的体外抗氧化活性。方法先用80%乙醇,然后用蒸馏水对楮实进行提取。其中醇提取物依次用石油醚、***仿、乙酸乙酯、正丁醇萃取,水提部分经醇沉、干燥后得到楮实粗多糖。运用羟基(·OH)自由基体系、二苯代苦味基肼(DPPH·)自由基体系和对Fe3+的还原能力实验,比较楮实各提取物的抗氧化活性。结果楮实提取物对·OH自由基和DPPH·自由基均有较好的清除作用,其中总醇提取物对·OH自由基的清除能力最强, mg/ml;乙酸乙酯提取物对DPPH·自由基的清除效果最好, mg/ml;各提取物对Fe3+均有较强的还原能力,乙酸乙酯提取物还原效果最为明显。结论楮实提取物具有一定的抗氧化活性,在抗衰老方面有广泛的应用前景。
【关键词】楮实;抗氧化活性;清除自由基;还原力
【Abstract】 Objective To study the antioxidant activities of extracts from Broussoia papyrifera in vitro. Methods B. papyrifera ether, chloroform, ethyl acetate, nbutanol. At the same time, crude haride . Results The extracts from B. papyrifera had good scavenging effect on the hydroxyl free radical and DPPH free radical. Ethanol extract had the best scavenging effect on hydroxyl free radical system, g/ml. Ethyl acetate extacts had the best scavenging effect on DPPH free radical, g/ml. All of the extracts had strong deoxidization ability of Fe3+. Among of them, ethyl acetate extracts had significant deoxidization ability. Conclusions Broussoia papyrifera has stronger antioxidant activities and 处有最大吸收。取各萃取物50 mg,用无水乙醇溶解并定容至25 ml;取粗多糖50 mg用蒸馏水溶解,定容至25 ml,配制得2 mg/ml的各样品质量浓度,再以该质量浓度配制成所需浓度。依次加入样品4 ml,9 mmol/L FeSO4 ml,9 mmol/ ml, mmol/L H2O2 ml。该体系于37℃水浴中加热30 min,于510 nm处测定样品吸光度(Ai)。用4 ml蒸馏水代替体系中的样品,测定得空白吸光度(A0)。 mmol/L H2O2,测定样品本底吸光度(Aj)。每个质量浓度样品做3