文档介绍：Tissue distribution and ics of 125I-Cry1Ab Abstract
Tissue distribution and ics of 125I-Cry1Ab
Abstract
Background: ically modified foods (GM foods or biotech foods) are foods that derived from ically anisms (GMOs) which have had specific changes introduced into their DNA by ic engineering techniques in order to add or enhance desired traits to them such as increased resistance to herbicides and insects or improved nutritional content. And it is traditionally considered that foods derived from ic modified plants pose no greater risk to human health than conventional food. However, more and more adverse phenomenon have occurred to human and animals in recent years. There is a possibility that introducing a gene into a plant create a new allergen or cause an allergic reaction in susceptible individuals. And there are appreciable differences in the intestines of rats fed on ically modified potatoes and those fed on unmodified potatoes. Also it is found that the reproductive capacity of hamster fed on ically modified soybean declined. All of this cases drive us carefully think about and assess the safety of ically modified food and feed. In all ically modified crops, transgenic Bt crops accounted for the vast majority, in which transgenic protein Cry1Ab is the most widely used and relatively stable. So we selected it as the representative of the transgenic protein to investigate its absorption, distribution and ics.
Objective: To investigate the bio-distribution and ics of Cry1Ab in plasma and to determine whether Cry1Ab can be absorbed through gastrointestinal tract and specifically concentrate in ans, in order to provide experimental evidence and data to assess the safety of ically modified food to human’s health.
Methods: (i) Cry1Ab was labeled with 125I using iodogen reagent and quality
control of the radiotracer was performed. (ii) The differences were observed of bio-distribution in BALB/c mice from two ways of drug delivery: gavage administration and caudal