文档介绍：高效液相色谱法测定生物样本中头孢噻肟和舒巴
:顾宜,王晓娟,王荣,白娟娟,高苏莉
【关键词】头孢噻肟;舒巴克坦;色谱法,高压液相;血浆;尿
【Abstract】 AIM: To establish a determination method of concentrations in human biologic sample folloinistration of cefotaxime and sulbactam. METHODS: A RPHPLC method ine cefotaxime and sulbactam in plasma and urine. The mobile phase of cefotaxime and sulbactam consisted of 1 g/L triethylamine (regulated ethanol in a 68∶32 and 84∶16 (v/v) respectively and pumped at a floL/min. The plasma samples ethanol, the urine samples n and monitored and 220 nm respectively. RESULTS: The assay of cefotaxime in human plasma and urine g/L and - mg/L respectively, it of 1 mg/L and mg/L respectively. The assay of sulbactam in human plasma and urine g/L, it of mg/L and mg/L respectively. The average recovery of the method e and sulbactam sample of plasma and urine stored in a -20℃ refrigerator became stable ethod is rapid, accurate and usable for the determination of cefotaxime and sulbactam concentrations in plasma and urine.
【Keye; sulbactam; chromatography, high pressure liquid; plasma; urine
【摘要】目的: 为静滴注射用头孢噻肟钠舒巴坦钠后在血浆和尿液中的药物浓度分析提供检测方法. 方法: 采用HPLC测定头孢噻肟钠血浆和尿液中浓度. 在血样中加入甲醇沉淀蛋白后,尿样用超纯水稀释后取上清液进样,色谱分析条件: 以C18反相柱作分析柱;柱温:室温;流速: 1 mL/min;头孢噻肟和舒巴坦的流动相分别为:1g/L的三乙***水溶液()∶甲醇=68∶32和84∶16;头孢噻肟和舒巴坦的紫外检测波长分别为254 nm和220 nm. 结果: