文档介绍：Proteomics
Bio-Rad蛋白质组双向电泳实验操作手册
ProteomeWorksTM System
双向电泳实验流程
样品制备(Sample preparation)
固相预制胶条的水化(IPG strip rehydration)
第一向等电聚焦(IEF)
胶条的平衡(IPG strip equilibration)
第二向SDS-PAGE电泳(SDS-PAGE electrophresis)
凝胶的染色及检测(Detection/Staining)
PDQuest软件分析(Software analysis)
质谱鉴定(Protein identification)
目录
实验材料
IPG预制胶条及载体***电解质
蛋白质定量试剂盒及其试剂
试剂盒及其试剂
化学试剂
蛋白质Marker
染色试剂
注意事项
第二章 SDS-PAGE聚丙烯酰***凝胶电泳
2. 1 溶液的配制
2. 2 SDS-PAGE凝胶的配制
2. 3 操作方法
2. 4 注意事项
第三章双向电泳
3. 1 溶液配制
3. 2 操作步骤
3. 3 注意事项
附录1 双向电泳完整的操作步骤
附录2 聚丙烯酰***凝胶电泳凝胶的配置
附录3 细胞样品的一般处理步骤
附录4 组织样品的一般处理步骤
第一章实验材料
IPG预制胶条及载体***电解质
(一)IPG预制胶条(美国Bio-Rad公司),-20℃冰箱保存
ReadyStrip IPG Strips, pH 3–10, 7 cm, 163-2000
ReadyStrip IPG Strips, pH 4–7, 7 cm, 163-2001
ReadyStrip IPG Strips, pH 3–10 nonlinear, 7 cm, 163-2002
ReadyStrip IPG Strips, pH 3–6, 7 cm, 163-2003
ReadyStrip IPG Strips, pH 5–8, 7 cm, 163-2004
ReadyStrip IPG Strips, pH 7–10, 7 cm, 163-2005
ReadyStrip IPG Strips, pH 3–10, 17 cm, 163-2007
ReadyStrip IPG Strips, pH 4–7, 17 cm, 163-2008
ReadyStrip IPG Strips, pH 3–10 nonlinear, 17 cm, 163-2009
ReadyStrip IPG Strips, pH 3–6, 17 cm, 163-2010
ReadyStrip IPG Strips, pH 5–8, 17 cm, 163-2011
ReadyStrip IPG Strips, pH 7–10, 17 cm, 163-2012
ReadyStrip IPG Strips, pH 3–10, 11 cm, 163-2014
ReadyStrip IPG Strips, pH 4–7, 11 cm, 163-2015
ReadyStrip IPG Strips, pH 3–10 nonlinear, 11 cm, 163-2016
ReadyStrip IPG Strips, pH 3–6, 11 cm, 163-2017
ReadyStrip IPG Strips, pH 5–8, 11 cm, 163-2018
ReadyStrip IPG Strips, pH 7–10, 11 cm, 163-2019
ReadyStrip IPG Strips, pH –, 17 cm, 163-2020
ReadyStrip IPG Strips, pH –, 17 cm, 163-2021
ReadyStrip IPG Strips, pH –, 17 cm, 163-2022
ReadyStrip IPG Strips, pH –, 17 cm 163-2023
ReadyStrip IPG Strips, pH –, 11 cm, 163-2024
ReadyStrip IPG Strips, pH –, 11 cm, 163-2025
ReadyStrip IPG Strips, pH –, 11 cm, 163-2026
ReadyStrip IPG Strips, pH –, 11 cm, 163-2027
ReadyStrip IPG Str