文档介绍：lactose conversion and the GOS concentration were % and , respectively. Na2B4O7 significantly improved the GOS yield.
Preparation of β-galactosidase CLEAs: The optimal conditions for preparation of β-galactosidase CLEAs were determined as 20 times dilution of original enzyme liquid, 1:1 of 2-propanol (v/v) precipitation for 30min at 4 °C, and cross-linking of 30min by % (v/v) glutaraldehyde. Thus % of the reservation activity could be otained. Compared with the free enzyme, the β-galactosidase CLEAs had wider range and higher value of pH and temperature, and excellent thermal stability.
Preparation of ic β-galactosidase CLEAs: The optimal conditions for preparation of ic β-galactosidase CLEAs were determined as follows: 100μL Fe3O4 ic nanoparticle and 2mL 20mg/mL BSA were mixed and stirred for ; 20 times dilution of original enzyme liquid, 1:1 of 2-propanol (v/v) precipitation for 60min at 4 °C, and cross-linking of 60min by % (v/v) glutaraldehyde. Thus % of the reservation activity could be otained. The ic β-galactosidase CLEAs could be easily recycled, so even if reused by four times, the yield was still 20% of the starting value.
KEY WORDS: β-galactosidase, galacto-harides, metal ions, sodium borate, ic nanoparticles, cross-linked enzyme aggregates
目 录
前言......................................................................................................................... 1
第一章文献综述................................................................................................... 2
功能性低聚糖.......................................................................................... 2
特性............................................................................................... 3
物理和化学性质........................................................................... 3
生理功能....................................................................................... 3
几种常见功能性低聚糖的性质及生产制备方法..