文档介绍：fermentation and the biological pesticides is 95‰1 00%and %respectively(the dosage is 8%).We can get the conclusion that the health—effects ofbiological pesticides are higher than the ,the biological pesticides have a longer shelf lifc,SO it can better meet the needs ofproduction and promotion.
the screening of application dose in and out of room,we got the best
proportion is 8%(quality than to the soil).
At the same time,after the measurement to the height and weight of cucumber and the
acidity and salinity of rhizosphere soil,we fond the product after solid fermentation can improve the nature of the soil and call promote the growth of ,the trend to reduce soil acidity iS clearer than to reduce soil this reflects the of the Trichoderma the future,they will have more potential for
wider application and promotion.
Key words: REMI;Trichoderma mutants;orthogonal design;Fermentation Process
缩写词表
Restriction Enzyme-mediated Integration 限制性内切酶介导整合
PEG Polyethylene Glycol 聚乙二醇
十六烷基三***溴化铵
ddH20 双蒸水
Deoxynucleotide Triphosphates 脱氧核苷三磷酸
Etllidium Bromide 溴化乙锭
Ethylenediamine Tetraacetic Acid 乙二***四乙酸
PCR Polymerase Chain Reaction 聚合酶链式反应
Tris Tris(Hydroxymethyl)Aminomethane 三(羟***)氨基甲烷
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本人声明所呈交的学位论文是本人在导师指导下进行的研究工作及取得的研究成果。据我所知,除了文中特别加以标注和致谢的地方外,论文中不包含其他人已经发表或撰写过的研究成果,