文档介绍：活血止痛胶囊含量测定方法探究
盐城市中药饮片有限公司刘祥(盐城 224002)
摘要:目的建立高效液相色谱法测定活血止痛胶囊中阿魏酸含量的方法。方法采用Agilent
C18柱(×150mm,5µm);乙***-%磷酸溶液(17:83)为流动相;流量 ;
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检测波长为 316nm;进样量为 10μl。结果进样量在 ~96µg*ml 浓度时与峰面积呈良好
线性关系,r=(n=5),平均回收率 %,RSD: %。结论该法简便、准确、
结果满意。
关键词:阿魏酸;高效液相色谱法;含量测定
HPLC Determination for Huoxuezhitong of Capsule
liuxiang
Yancheng Neautus Traditional Chinese Medicine .(Yancheng224002)
Abstract:Objective To develop a method for detemination for Huoxuezhitong of Capsule by
HPLC. Methods The Agilent C18 (×150mm,5µm) was used, Acetonitrile-
%Phosphoric acid(17: 83) as the mobile phase, the flow rate was ·min-1, the detection
wavelength was 316nm. The injected quantity of 10μl. Results The linearity of peak area was
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good when the injected quantity of Granisetion was in the range of 20~200µg*ml (r=
,n=5), The recovery of this method was %,RSD: %. Conclusion This
method is convenient,accurate and the results of experiment satisfactory.
Keywor of .
活血止痛胶囊主要有效成分是当归、三七等(以阿魏酸为指标,标准编号:
WS3-084(Z-74)-95(Z));作用是活血散瘀,消肿止痛。用于跌打损伤,瘀血肿痛。因该主药
含量测定方法是薄层扫描法测定[1],因薄层扫描法测定操作繁琐且费时,给检验带来了不便,
重现性差。查阅文献[2]并加以摸索,建立了高效液相色谱法,适合于活血止痛胶囊主要有效成
分是当归等,原标准是以阿魏酸为指标的含量测定[1]。使之简便、准确、结果满意。
1、仪器与试药
仪器: G1311A 四元高效泵液相测定仪,G1314B 紫外检测器,Agilent1200 色谱工作站,
G1322A 真空脱气机,G1316A 柱