文档介绍：Coh阿】TS
CoNTENTS
Abstract in Chinese .1
Abstract in English. ................... .... . . .... ...... ..III
1 Introduction . ..1
Research background ..1
The research purpose and meaning l
Research progress at home and abroad 2
7I'lle role anisms in soil ecosystem 2
Soil munity diversity ofresearch methods ..3
In soil microbial change under different soil using the way ofrelated research ..5 The research content and the technical route 5
The research content 5
"rechnical route . .6
:}Materials and Methods . . ..7
The study area . 7
Tlle northeast black earth area 7
Test area geographical location and climate and hydrological conditions 7
TESt setting . ...8 2.!;1rhe tested soil .8
..................................................9 Against determination ofthe number of soil culturable anisms 9
munity diversity Biolog microplate method .10
Soil DNA extraction and quantitative PCR analysis ..11
454 high-throughput sequencing technologies .12
3 The results and analysis .13
Different soil culturable microbial quantity using way .13
Different soil using Biolog analysis results 14
munityOiltheusing characteristicofallkinds ofcarbon source 16
Quantitative PCR results .17
Track 16 S bacteria and archaea 16 S biomass variation characteristics 17
Ammonia oxidation bacteria and ammonia oxidation archaea biomass variation characteristics . . ..17
454 hi曲-throughput sequencing classification results 18
Track ofdoor(phylum】level classification 18
munity diversity index ..20
munity structure change 22
4 Discussion . .:!zI
IIl
万方数据
东北农业大学农学硕士学位论文
!;IConclusion . . . . ... . . . . . . . . . . . . . ... . . ... . . . . . . . ..:16
Acknowledgement. . . . ... . . . . .. .. . ... . . ....... ................................... .27 References. . . . . . . . . . . . . . .