文档介绍：高效液相色谱法测定散寒解热颗粒剂中麻黄碱的含量
林妮王鹤东
(辽宁省医药工业研究院,沈阳110015)
摘要目的:建立散寒解热颗粒剂中麻黄碱的含量测定方法。方法:样品用甲醇超声提取,采用高效液相色谱法,Nucleosil-C18(200mm×,7µm)分析柱,乙腈-(用10%)(:)为流动相,检测波长215nm。结果:。,~(r=),%(n=5)。结论:方法简便,快速,结果准确可靠,可作为散寒解热颗粒剂中麻黄碱的含量测定方法。
关键词:散寒解热颗粒剂;麻黄碱;高效液相色谱法;含量测定
HPLC determination of Ephedrine in Sanhanjiere granules
LinNi Wanghedong
(Liaoning Institute of Pharmaceutic Industry, Shenyang110015)
Abstract Objecetive:To establish a method for determining the concentration of ephedrine in Sanhanjiere :The sample was extracted with methanol on a was used for the determination of ephedrine in Sanhanjiere chromatographic procedure was carried out using Nucleosil-C18(200mm×,7µm) as an analytical column and a mixture of volume of acetonitrile, volume of water which was contained Sodium dihydrogen phosphate and adjusted the solution by phosphoric acid as mobile detection wavelength was set at : The peaks of ephedrine appeared on about minutes and the resolutions of them were more than linear ranges were between and (r=),with the average recoveries of %(n=5).Conclusion:This method is sensitive,quick and accurate for the measurement of puerarin in Sanhanjiere gr