文档介绍：CHAPTER 15
BIOPHOTONICS APPLIED TO PROTEOMICS
MICHEL FAUPEL, DÉBORA BONENFANT, PATRICK SCHINDLER,
ERIC BERTRAND, DIETER MUELLER, MARKUS STOECKLI,
FRANCIS BITSCH, TATIANA ROHNER, DIETER STAAB,
and JAN VAN OOSTRUM
Novartis Institutes for Biomedical Research, Basel
Table of Contents
Abstract ............................................................................. 323
1. Introduction ..................................................................... 323
2. Two-Dimensional Electrophoresis ............................................. 324
3. Proteomics Analysis by Mass Sectrometry .................................... 325
4. Mass Spectrometry Imaging.................................................... 329
. Principles and Methods ................................................... 330
. Compound Imaging ....................................................... 331
. Peptide Imaging ........................................................... 332
. Protein Imaging ........................................................... 333
5. Conclusions ..................................................................... 336
References .......................................................................... 337
Abstract: Since pletion of the human genome sequencing, our understanding of gene and
protein function and their involvement in physiopathological states has increased dramati-
cally, partly due to technological developments in photonics. Photonics is a very active area
where new developments occur on a weekly basis, while established tools are adapted to
fulfill the needs of other disciplines like genomics and proteomics. Biophotonics emerged
at the interface of photonics and biology as a very straightforward and efficient approach to
observe and manipulate living systems. In this chapter, we review the current applications
of photonics and imaging to proteomics from 2D gels analysis to molecular imaging.