文档介绍：2011届分类号:
单位代码:10452
毕业论文
槐米中芦丁超声法和碱溶酸沉法提取比较
姓名刘义涛
学号 200709820229
年级 2007级
专业制药工程
系(院) 生命科学学院
指导教师张海娟
二〇一一年五月
槐米中芦丁超声法和碱溶酸沉法提取比较
Comparation of Ultrasonic and Alkali solution and acid precipitation extracted Rutin from Sophora japonica
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姓名刘义涛
专业制药工程
指导教师张海娟
临沂大学
二〇一一年五月
摘要
槐花中主要成分为芦丁,又名芸香苷,现在报道的提取方法有很多,本实验主要研究超声提取法与碱溶酸沉法,比较这两种方法提取率高低。超声法是用70%乙醇溶液浸泡槐米粉,在超声清洗器中进行超声处理30min,处理2次,收集滤液,用浓盐酸调节PH值至3,静置,至沉淀完全后,抽滤,收集沉淀得到芦丁粗品,粗品在60-70℃水浴中溶解,煮沸15min,趁热抽滤,静置滤液,待沉淀完全,抽滤,沉淀在65℃干燥40min,得到芦丁的纯化物;碱溶酸沉法是用蒸馏水浸泡,加氧化钙1-,调节PH至8,加热至微沸,保持40min,趁热过滤,浸煮2次,收集滤液,用浓盐酸调节PH至5,静置至沉淀完全,将沉淀在60-70℃水浴中,溶解,煮沸15min,趁热抽滤,静置滤液,待沉淀完全,抽滤,沉淀在65℃干燥40min,得到芦丁的纯化物。本实验中碱溶酸沉法比超声法的提取率高。
关键词:槐米;超声提取法;碱溶酸沉法;紫外分光光度法;聚酰***薄膜
ABSTRACT
This pared ultrasonic extraction with alkali solution and acid precipitation in extraction rate of rutin. Ultrasonic method is soaked Sophora japonica with 70% ethanol, the ultrasonic cleaner carried out the Sophora japonica with 30 minutes .then filtering the solution and carried out the residue in the ultrasonic cleaner again. Collecting the filtrate in beaker and Adjusting the filter’s PH to 3 with concentrated hydrochloric acid. Standing the filter and pletely. Collecting the precipitate by the filtration. The precipitate is crude extract rutin. Recrystallizating the crude extract rutin in 60-70 ℃ water bath and boiling the recrystallizate’s solution on electric furnace with 15 minutes. Filtrating the solution when it is boiling and the filtrate aside until the pletely. Filtrating the solution, Then collecting the precipitate. The precipitate dried at 65 ℃ in Vacuum oven with40 minutes . Getting the purified rutin. Alkali solution and acid precipitation is soaked Sophora japonica with distilled water, Adding calcium oxide g. adjusting the PH to 8, Boiling the Solution with 30 minutes, then filtering the solution and carried out the residue again, Collecting the filtrate in beaker and Adjusting the filter’s PH to 5 with concentrated hydrochlor