文档介绍：阻断 hedgehog 信号通路对 细胞
增殖和迁移的影响摘 要
目的:、迁移中的作用。方法:,用5μmol/L、15μmol/L、25μmol/L环耙明处理 、48h、72h,采用MTT检测环耙明对细胞增殖的影响;EDU法检测细胞DNA合成情况;划痕试验和transwell实验检测细胞迁移能力;Real-timePCR法检测细胞中Gli1、MMP-9 mRNA表达情况。结果:用5μmol/L、15μmol/L、25μmol/、 48h、72h后,MTT检测结果显示细胞增殖速度减慢,与空白组相比差异具有统计学意义(p<);EDU法检测结果显示25μmol/L的环耙明作用于细胞24h、48h、72h后, (p<);划痕试验结果显示,15μ mol/L、25μmol/L浓度的环耙明能抑制细胞的迁移,与对照组相比差异具有统计学意义(p<);Transwell实验结果显示,5μmol/L 、 24h后,穿膜细胞数与对照组相比明显减少(p<);Real -time PCR法实验结果显示5μmol/L、15μmol/L、25μmol/L环耙明处理组与对照组相比,Gli1、MMP-9 mRNA 表达水平明显降低(p<)。结论:,;并能降低细胞的迁移能力,、MMP-9 mRNA 的表达水平下降有关。
关键词:Hedgehog信号通路;肝癌;增殖;迁移
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The role of Hedgehog signaling pathway in proliferation and immigration of cell
Abstract
Objective:To investigate the roles of Hedgehog signaling pathway in prolifera- tion and immigration of cell.
Methods: HepG2, cells were divide into blank group,and the low, median and
High concentration of cyclopamine treatment groups,which was treated with the Concentrations of cyclopamine were 5μmol/L,15μmol/L,25μmol/L,respectively. After
24h,48h,72h, The proliferation of cell was detected by cellular DNA synthesis were detected by EdU. The invasive ability was measured by Boyden chamber invasive migration ability was measured by scratch test. The expression levels of Gli1,MMP-9 mRNA were measured by rea1-time PCR.
Results:1. MTT assay results showed that the cyclopamine treatment groups with the
concentration of 5 μ mol/L、15μmol/L、25 μ mol/L was significantly reduced cell pared with the control group,which the difference was statistically significant (p<). detection results showed the cyclopamine treatment groups decreased the cellular DNA pared with the control group, the diffe