文档介绍：GEL ELECTROPHORESIS
Reference: Sambrook et al. (1989). Molecular Cloning Manual.
Solutions Needed:
Agarose
1X TAE buffer
10mg/mL Ethidium Bromide (EtBr)
6X Loading Dye containing xylene cyanol and bromophenol blue dyes
Apparatus Needed:
Gel cast
Gel box
Cables
b
Plastic (Saran) wrap
GEL ELECTROPHORESIS
Measure out X grams of agarose (powder) depending on the final percentage of agarose in the gel.
Example: If you want to make a 1% agarose gel (1 g/100 mL, w/v), weigh out 1g of agarose for 100 mL of agarose solution
Carefully, put the agarose in a 250-mL Erlenmeyer flask.
Measure out 100 mL of 1X TAE buffer using a plastic or glass graduated cylinder.
Add 100 mL of 1X TAE buffer into the flask in step 2.
Cover the flask with a piece of plastic wrap. Poke 3-4 holes on the plastic wrap using a pointed end of a pencil or pen (note: the holes allow the steam to escape during microwaving in step 6 below). Swirl the solution to break up any lumps of agarose granules.
Microwave the solution for about 2 minutes or until the agarose granules pletely melted.
Be careful with the flask. The solution gets very hot.
Constantly watch over the solution because when it starts boiling, it might overflow.
Swirl gently the solution several times while microwaving to help melt agarose evenly.
Once the agarose has pletely, the solution is clear.
Once the agarose has pletely,