渤海海洋沉积物放线菌非培养多样性研究.doc

文档介绍：渤海海洋沉积物放线菌非培养多样性研究
摘要
放线菌研究在酶技术、医药工业和环境保护领域有重要的应用价值有广阔的应用前景, 研究渤海沉积物中的放线菌非培养多样性,能促进放线菌资源开发,有利于新物种和新产物的发现。
利用放线菌特异性引物扩增16S rDNA基因,构建16S rDNA基因克隆文库,对文库中的克隆片段进行RFLP(Restriction Fragment Length Polymorphism,限制性片段长度多态性)分析。研究分析表明:B71-2样品的27个克隆子获得了23个OTUs, 其中 %的克隆序列分布于放线菌门(phylum Actinobacteria)放线菌亚纲(Actinobacteridae)和酸微菌亚纲(Acidimicrobidae)中,%的克隆序列属于放线菌的新分类单元;另外 %的克隆序列以极高的自展值在放线菌门内支持形成一个独立的大分支,极有可能代表一个新亚目或更高级分类单元的类群。B37-3样品的31个克隆子获得了24个OTUs,其中 %的克隆序列分布于放线菌门(phylum Actinobacteria)放线菌亚纲(Actinobacteridae)和酸微菌亚纲(Acidimicrobidae)中,%的克隆序列属于放线菌的新分类单元;另外 %的克隆序列以极高的自展值在放线菌门内支持形成一个独立的大分支,极有可能代表一个新亚目或更高级分类单元的类群。
通过分析免培养结果,表明渤海放线菌具有较高的多样性,并蕴藏着多种新分类单元,有进一步研究价值。
关键词:免培养方法放线菌多样性渤海海洋沉积物
Studies on the diversity of uncultured Actinomycetes from the sediment of Bohai sea
Abstract
The study of Actinomycetes has potential application in the fields of medical industry, biological chemistry and environmental protection. Studies on the actinobacterial diversity in the Yellow sea and Bohai sea would promote the development and utilization of actinomycetes resources.
Specific primers were used to amplify the actinobacterial 16S rDNA gene, and corresponding clone libraries were constructed for the sediment samples. Different clones selected on the basis of HaeIII digestion patterns were sequenced. The analysis of 16S rDNA gene sequences showed that: 27 clone sequences of B72-1include 23 OTUs, % of which belonged to phylum Actinobacteria,subclass Actinobacteridae and subclass Acidimicrobidae . In these two subclasses, % of the clone sequences belonged to new taxons of Actinomycetes . % of the clone sequences with high bootstrap values ​​in phylum Actinobacteria support the formation of an independent branch, most likely represents a new suborder, or more advanced taxa groups. 31 clone sequences of B37-3 include 24 OTUs ,% of which belonged to phylum Actinobacteria,subclass Actinobacteridae and subclass Acidimicrobidae. In these two