文档介绍：Effect of nimesulide on chemoprevention of chronic atrophic
gastritis and expression of P 16 in stomach mucous
membrane
ABSTRACT
Objective: This study was designed to construct the rat model with chronic atrophic
gastritis (CAG), and observe the preventive effect of cyclooxygenase (COX)-2
inhibitor nimesulide on CAG rats, then try to investigate the molecular mechanism to
provide theoretical evidence for the chemical prophylaxis of chronic atrophic
gastritis .
Methods: 72 male rats (6-week) were divided into 6 groups, each group drinked
different drugs: control group (normal); CAG model; nimesulide group with different
dose (4mg, 6mg, 8mg per rat); positive group (folacin). Rats' consciousness, appetite,
glossiness of clothing hair, sedes and body weight were observed. All the rats were
killed after 24 weeks' breed, their stomach mucous membrane were viewed by HE
staining; The expression of COX-1, COX-2 and P16 protein in mucous membrane
epithelioid cells were detected by immunohistochemistry. Data from each experiments
were expressed as mean±SD. Measurement data were analyzed by F test and
enumeration data were by χ2 test by using SPSS software, P< considered
significant.
Results: There were 66 rats remaining after 24 weeks' feeding. The CAG incidence
rate was significant difference than control in model group (P<), indicated that
the model was established essfully. The body weight of model rat was lower than
normal group, nimesulide and folacin group (P<), there is no significant
difference between other groups (P<). As for the precaution, nimesulide group
and positive group could prevent CAG (P<), but there was no significant
difference between different dose of nimesulide group and positive group (P>).
6
COX-1 were expressed in each groups, and there was no difference between them.
The expression level of COX-2 and p16 protein were different between model and
nimesulide groups (P<), but there is no