文档介绍：中国农学通报 2011,27(19):239-243
Chinese Agricultural Science Bulletin
甘蔗健康苗圃宿根矮化病检测及结果分析
刘睿,沈万宽,陈仲华,杨湛端,邓海华
(广州甘蔗糖业研究所/广东省甘蔗改良与生物炼制重点实验室,广州 510316)
摘要:甘蔗宿根矮化病(Ratoon Stunting Disease,RSD)是中国蔗区的一种主要甘蔗病害,影响甘蔗产
量、蔗糖分及宿根蔗年限,也是导致甘蔗品种种性退化的重要原因之一,具有较严重的经济危害性。笔
者应用实验室建立的甘蔗宿根矮化病菌 PCR 检测技术对 1 级或 2 级健康种苗圃种苗进行甘蔗宿根矮化
病监测,以明确各级种苗圃宿根矮化病发生情况,为指导生产提供科学数据。检测结果为‘粤糖00-236’
和 ROC22 新植 1 级和 2 级健康种苗圃均未检测出 RSD,宿根 1 级健康种苗圃 RSD 检测率分别为 20%和
5%,各级健康种苗圃 RSD 总检出率分别为 %和 2%,分别较其普通种茎苗 RSD 检出率降低 %和
68%。结果表明,建立的甘蔗健康种苗圃已有效地去除了甘蔗RSD病菌。
关键词:甘蔗;健康种苗;宿根矮化病;PCR
中图分类号: 文献标志码:A 论文编号:2011-0569
Occurrence of Sugarcane Ratoon Stunting Disease in Sugarcane Healthy Seed Nurseries
Liu Rui, Shen Wankuan, Chen Zhonghua, Yang Zhanduan, Deng Haihua
( /
Guangzhou Sugarcane Industry Research Institute
, Guangzhou 510316)
Guangdong Key Lab of Sugarcane Improvement and Biorefinery
Ratoon stunting (RSD) is a major disease of sugarcane affecting sugarcane yield, sucrose and ratoon
Abstract:
sugarcane years, and one of the important reasons of sexual degradation, causing seriously economic loss. In
this paper, in order to investigate the occurrence of ratoon stunting disease at all levels and to provide scientific
guidance for the nursery plantlets production, an established ratoon stunting virus PCR detection technology
was used to monitor ratoon stunting disease-free seed produ