文档介绍：莽山烙铁头蛇粗毒的的生物学活性
刘宇, 聂东宋﹡, 吴喆,刘立超,朱政斌
(湖南理工学院化学化工系,中国岳阳 414000)
摘要: 莽山烙铁头蛇是我国濒临灭绝的物种,对其毒素的深入研究可以发现新的生物活性分子和及其的作用机理,也为莽山烙铁头独特的分子进化机制提供理论依据。本文分析了莽山烙铁头蛇毒粗毒对小鼠的半致死量,并采用膜片钳全细胞记录技术,研究了莽山烙铁头蛇毒粗毒对大鼠背根神经节神经元河豚毒素不敏感(TTX-R)钠通道、河豚毒素敏感(TTX-S)钠通道、低电压激活(LVA)电压依赖性钙离子通道和高电压激活(HVA)钙离子通道的作用。结果表明: mg/kg, 粗毒对钠通道和钙离子通道作用存在明显差异,10、100、1000 mg/L的莽山烙铁头蛇毒素对河豚毒素不敏感(TTX-R)钠电流有抑制作用,而100mg/L的莽山烙铁头蛇毒素对河豚毒敏感型(TTX-S) 钠电流无明显影响; 100、1000mg/L 的莽山烙铁头蛇毒素对HVA型钙离子通道有抑制作用;1000mg/L 的莽山烙铁头蛇毒素对LVA型钙离子通道电流峰值的有抑制作用,本研究为莽山烙铁头蛇毒素功能的深入研究奠定了良好的基础。
关键词莽山烙铁头蛇;粗毒;膜片钳全细胞记录;生物学活性。
Study on the biological activity of crude venom from the Ermia mangshanensis
LIU Yu, NIE Dongsong﹡, WU Zhe, LIU Lichao,ZHU Zhengbin
(Hunan Institute of Science and Technology; Chemistry and Chemical Engineering College, Yueyang, China, 414000)
Abstract: Ermia mangshanensis snake is an endangered species, in-depth study on its toxins can not only discover new biologically active molecules and its mechanism, but also provide a theoretical basis for its unique mechanism of molecular evolution. In this study, the cell patch-clamp technique was uesed to study the crude venom on the effect of rat root ganglion neurons back TTX -R sodium channels、TTX - S-type sodium channels、Calciumion channels HVA type and Calciumion channels LVA type. The Results showed that 10,100,1000 mg / L of venom significantly inhibited TTX-R sodium channel and th