文档介绍：碱性成纤维细胞生长因子促进兔缺血后肢血管新生的
【摘要】目的:了解局部应用碱性成纤维细胞生长因子(bFGF)对促进兔缺血后肢血管新生的作用。方法:25只日本大耳白兔随机分2组,外科结扎切断各兔股动脉及其分支,制作兔后肢缺血模型。试验组各兔在缺血后肢肌肉内多次注射重组人bFGF蛋白(n=15);对照组给予等剂量生理盐水(n=10)。术后4周,各兔行腹主动脉造影观察侧支血管形成情况,取内收肌和腓肠肌肌肉行病理切片HE染色应用图像分析系统统计血管密度,并用免疫组织化学方法检测内收肌和腓肠肌中VEGF阳性表达的血管数。结果:试验组兔侧支循环血管条数、血管密度及VEGF阳性表达的血管数均大于对照组(P<),缺血状态得到改善。结论:在兔缺血后肢肌肉中注射bFGF蛋白可促进血管新生,增加兔缺血后肢血液灌注,改善肢体缺血状态。【关键词】缺血&#12539;成纤维细胞生长因子&#12539;新生血管化,生理性&#12539;兔
Basic fibroblast groproves the angiogenesis of the ischemic limbs in rabbits
【ABSTRACT】Objective:To evaluate the effect of local application bFGF on angiogenesis in ischemic limbs of :After ischemia models oral artery in each rabbit,rabbits ized to received intramuscular injection of rebinant human bFGF(n=15)or normal saline(n=10)in ischemic ia models inal aortography,histopathologic analysis and immunohistochemical studies ber of capillaries,the pensatory circulation blood vessel and the vascular endothelial cells that expressed VEGF protein in bFGF group is more than those of saline group(P<).Conclusion:Intramuscular injection of rebinant human bFGF can improve the angiogenesis of the ischemic limbs is rabbits.
【KEY ia&#12539;Fibroblast grog/kg)获得麻醉效果后,无菌条件下经左侧腹股沟韧带中点(摸到股动脉搏动后)做一纵形皮肤切口2cm,暴露股动脉后, 自股动脉起始部分分离股动脉,在股动脉起始处,用1号丝线结扎并切断,制成兔后肢缺血模型。
实验方法兔后肢缺血模型备制成功3h后,试验组各兔给予bFGF2000U(约5μg,用生理盐水溶解成2ml),分5点在股动脉结扎处下方肌肉内注射。按此法第3、5、7天各给药1次,共