文档介绍：反相高效液相色谱法同时测定参苓白术颗粒中白术内酯Ⅱ和白术内酯
作者:葛建,楼纪东,林芳,黄丽红,姜威,李明揆,卢秀莲
【摘要】目的建立一种同时测定白术内酯Ⅱ和白术内酯Ⅲ的高效液相色谱检测方法,并分析中成药参苓白术颗粒中白术内酯Ⅱ和白术内酯Ⅲ的含量。方法样品采用液-液萃取,以乙腈-水(50∶50)为流动相, ml· min-1,采用SHIMADZU ODS柱(150 mm× mm; 5 μm)色谱柱分离;检测波长220 nm。~ μg·ml-1,线性关系良好(r>), μg/ml,回收率98%以上,方法的稳定性、重复性和精密度均较好(RSD<3%)。结论该法操作简便、快速、灵敏、准确,已经成功地用于中成药参苓白术颗粒中白术内酯Ⅱ和白术内酯Ⅲ的含量测定。
【关键词】白术内酯Ⅱ白术内酯Ⅲ高效液相色谱-紫外检测法参苓白术颗粒
Abstract:ObjectiveTo develop an HPLC-UV method for the simultaneous determination of atractylenolide II and III and to detect the content of atractylenolide II and III in Chinese medicinal a simple liquid-liquid extraction, atractylenolide II and III n( mm×150 mm, 5 μm) and detected by UV spectrometry. The detection . The mobile phase consisted of acetonitrile-l·min-1. The content of atractylenolide II and III in Chinese medicinal preparation(Shenling Baizhu Granule) ultaneously determined by the method above after simple liquid-liquid linear calibration curves l-1(r>). The loit of quantification l-1. The recoveries ore than %, and the stability, repeatability and precision of the method ethod is specific, simple, sensitive and accurate. And this method ultaneously quantitate atractylenolide II and III in Chinese medicinal preparation (Shenling Baizhu Granule).
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