文档介绍：浙江万里学院
毕业设计(论文)
题目乐昌含笑组织培养过程中根的诱导研究
姓名俞慧丽
学号 03011970
系别生物与环境学院
专业生物技术034
指导教师吴月燕
摘要
以乐昌含笑离体培养得到的无根苗为试验材料,采用5因素4水平的正交设计,研究不同因素不同水平对乐昌含笑诱导生根的影响。结果表明: NAA对乐昌含笑生根作用影响最大,其次是IBA,再次是MS培养基,而IAA和6-BA对诱导生根的作用很小;, mg/LIBA,1/2MS,,-BA;最佳生根培养基组合为1/2MS++ mg/LIBA;并以最佳组合为生根培养基,研究不同添加物(维生素C、6-BA、维生素E、活性碳)对乐昌含笑生根诱导的影响,发现添加物活性碳(AC)和维生素E对生根诱导有明显的促进作用。
关键词:乐昌含笑;组织培养;诱导生根
ABSTRACT
Seedlings without root of Michelia chapensis from tissue culture were used to investigate the effect of different hormones and sucrose in the medium on induction of root with the orthogonal design L16(45). The results showed that the effect of NAA on rooting was the most obvious, next was IBA, then MS medium, but the effect of IAA and 6-BA was quite small. The best levels of different hormones were , mg/L IBA, 1/2 MS, IAA, and 6-BA, respectively. The growth of roots was best on 1/ 2MS medium with the addition of mg/L NAA + mg/L IBA. Then, take 1/2MS medium with the addition of mg/L NAA + mg/L IBA. Meanwhile, the effects of different additives (vitamin C, 6-BA, vitamin E and AC) in the sample on induction of root were also studied in this paper. The results demonstrated that the addition of AC and vitamin E were good for rooting induction.
Key Words:Michelia chapensi; tissue culture; rooting induction
目录
1 前言 1
2 材料与方法 2
材料 2
方法 2
材料消毒 2
试验设计 3
不同基本培养基浓度与不同激素种类和浓度的试验 3
不同添加物的试验 3
暗培养的试验 3
统计方法 5
3 结果与分析 5
不同基本培养基浓度与不同激素种类和浓度对诱导生根的影响 5
不同基本培养基浓度对诱导生根的影响 7
不同激素种类和浓度对诱导生根的影响 8
NAA对诱导生根的影响 8
IBA对诱导生根的影响 9
IAA对诱导生根的影响 9
6-BA对诱导生根的影响 9
不同添加物质对诱导生根的影响 9
活性碳(AC)对诱导生根的影响 10
维生素E对诱导生根的影响 10
维生素C对诱导生根的影响 11
6-BA对诱导生根的影响 11
暗培养对诱导生根的影响 11
4 结论与讨论 11
参考文献 14
致谢 15
附录1 外文翻译 16
附录2 文献综述 27
1 前言
乐昌含笑又名景列含笑,属木兰含笑属,常绿乔木,为南方的兰花,是含笑属中较为耐寒的常绿树种。其树形壮丽,树干通直,分枝均匀,枝叶茂