文档介绍：Gene Mapping & Cloning
Medical ics
Determination of the relative positions of genes on a DNA molecule (chromosome) and of the distance, in linkage units or physical units, between them.
Gene Mapping
1911, red-green color blindness localized to chromosome X
1968, Duffy Blood Group localized to chromosome 1
Linkage Analysis
A mathematical procedure that analyzes meiotic bination frequencies between pairs of genes to determine whether two loci are linked and, if so, how closely.
Linkage Analysis
A mathematical procedure that analyzes meiotic bination frequencies between pairs of genes to determine whether two loci are linked and, if so, how closely.
bination fraction
an, cM
ic Marker
A ic marker is a tag which allows to identify a place in a genome (locus), is a ‘tag’ near or within a gene of interest. ic markers are usually highly polymorphic.
ic Marker
Blood group; Serum protein; HLA
DNA RFLP
DNA STR & DNA VNTR
Single nucleotide polymorphism, SNP
Linkage Analysis
EcoRⅠ
EcoRⅠ
EcoRⅠ
kb
kb
Probe
Neurofibroma, NF1
RFLP
Ⅰ
Ⅱ
Ⅲ
1 2
1 2 3 4 5 6 7 8
1 2 3 4 5 6 7 8
Linkage Analysis
Neurofibroma, NF1
RFLP
Ⅰ
Ⅱ
Ⅲ
1 2
1 2 3 4 5 6 7 8
1 2 3 4 5 6 7 8
kb
kb
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