文档介绍：内蒙古大学硕士学位论文区域之间的DNA***化水平具有很大的差异。因此,本文提出利用离散增量法和欧式距离法来识别差异***化基因(DMG),并发现了一些***化差异显著的
基因。词时,针对管家基因的分析表明,管家基因保持着低***化水平,这对、保持细胞的基本代谢是至关重要的。
进一步,为了阐明CpG位点***化程度与邻近序列的相关性,以CpG为中心提取长度为23bp的核苷酸片段,构建CpG邻近序列数据库。根据CpG位点***化水平的不同,等间隔地分为11组,并对每组序列进行分析,结果显示CpG 位点***化程度与上下游DNA碱基组分相关联,低***化水平的CpG位点邻近序列有更多的C(c),G(,GG,GC,CG二联体。通过MEME motif 软件分析高、低***化序列集合,分别得到一些特定的模体(motif)。通过计算序列能量,相对稳定和灵活性,我们发现其能量、相对稳定和灵活性随着CpG位点***化程度的增加而逐渐减少。
关键词:DNA***化;MEMEmotif分析;转录终止区域;管家基因
万方数据
内蒙古大学硕士学位论文
THE ANAI。YSIS OF GENE METHYLATION DIFFERENCE IN DIFFERENT CELL LINES AND TISSUES AND THE CORRELATION BETWEEN SEQUENCE
COMPOSITION
AND CpG METHYLATION LEVEL ABSTRACT
DNA methylation is one of the most studied ic is a chemical process including a methyl group is attached on the number 5 carbon of the cytosine pyrimidine ring catalyzed by DNA methytransferases which mon in a 5'-’dinucleotide(called CpG).Most vertebrate genomes exist clusters of
methylated cytosine nucleotides,mainly in gene 5’non-coding methylation status of cytosines in CpGs influences protein—DNA interactions and chromatin structure and stability,and consequently plays a vital role in the regulation of biological processes such as transcription,X chromosome inactivation,genomic studies revealed that DNA methylation patterns in mammals are tissue-or cell line-,our understanding of the regulatory role of those specific DNA methylations remains ,the analysis and
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万方数据
内蒙古大学硕士学位论文
comparison of gene methylation characteristic between tissues,cell types is essential
for understanding the mechanism of DNA methylation in different biological
Based on Refseq gene annotation downloaded from the UCSC Genome Browser,21740 genes with more than two exons were extracted from total 4961 1 genes in RefSeq Bisulfite-Seq data downloaded from NCBI Database Browser and the value denotes the methylation degree for each CpG site is between 0
and any gene