文档介绍：Appl Microbiol Biotechnol (2011) 91:425–434
DOI -011-3296-1
ENVIRONMENTAL BIOTECHNOLOGY
A diverse munity in an anoxic
quinoline-degrading bioreactor determined
by using pyrosequencing and clone library analysis
Xiaojun Zhang & Siqing Yue & Huihui Zhong &
Weiying Hua & Ruijia Chen & Youfang Cao & Liping Zhao
Received: 29 January 2011 /Revised: 26 March 2011 /Accepted: 27 March 2011 /Published online: 3 May 2011
# Springer-Verlag 2011
Abstract There is a concern of whether the structure and library. Our results demonstrate that pyrosequencing of
diversity of a munity can be effectively the V3 region of the 16S rRNA gene is not only a powerful
revealed by short-length pyrosequencing reads. In this tool for discovering low-abundance bacterial populations
study, we performed a munity analysis on a but is also reliable for dissecting the munity
sample from a high-efficiency denitrifying quinoline- structure in a wastewater environment.
degrading bioreactor pared the results generated
by pyrosequencing with those generated by clone library Keywords Quinoline degradation . munity .
technology. By both technologies, 16S rRNA gene analysis Pyrosequencing . Clone library. 16S rRNA gene
indicated that the bacteria in the sample were closely related
to, for example, Proteobacteria, Actinobacteria, and Bacter-
oidetes. The sequences belonging to us were the