文档介绍：ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, Aug. 2010, p. 3086–3092 Vol. 54, No. 8
0066-4804/10/$ doi:-09
Copyright © 2010, American Society for Microbiology. All Rights Reserved.
Evaluation of a DNA Microarray, the Check-Points ESBL/KPC Array,
for Rapid Detection of TEM, SHV, and CTX-M Extended-Spectrum
-Lactamases and KPC Carbapenemasesᰔ
T. Naas,* G. Cuzon, H. Truong, S. Bernabeu, and P. Nordmann
Service de Bacte´riologie-Virologie, INSERM U914: Emerging Resistance to Antibiotics, Hoˆpital de Biceˆtre, 94275 Le Kremlin-
Biceˆtre, and Assistance Publique-Hoˆpitaux de Paris, Faculte´deMe´decine Paris-Sud, Paris, France
Received 11 September 2009/Returned for modification 13 December 2009/Accepted 1 June 2010
Extended-spectrum ß-lactamases (ESBLs) and Klebsiella pneumoniae carbapenemases (KPC carbepen-
emases) have rapidly emerged worldwide and require rapid identification. The Check-Points ESBL/KPC array,
a mercial system based on ic profiling for the direct identification of ESBL producers (SHV,
TEM, and CTX-M) and of KPC producers, was evaluated. Well-characterized Gram-negative rods (Entero-
bacteriaceae, Pseudomonas aeruginosa, obacter baumannii) expressing various ß-lactamases (KPC-2, SHV,
TEM, and CTX-M types) were used as well as wild-type reference strains and isolates harboring ß-lactamase
genes not detected by the assay. In