海州香薷中铜积累与生长特性及铜耐性之间的关系研究.doc

文档介绍：
海州香薷中铜积累与生长特性及铜耐性
之间的关系研究#
张红晓1,宋玉峰2,张芬琴3*
5
10
15
20
25
30
35
40
(1. 河南科技大学农学院,河南洛阳 471000;
2. 南京农业大学生命科学学院,南京 210095;
3. 河西学院农业与生物技术系,甘肃张掖 734000)
摘要:以耐铜植物海州香薷(Elsholtzia haichowensis Sun)为材料,研究水培条件下不
同浓度 CuSO4 处理 7 天后,海州香薷根、茎、叶中铜积累量与其生物量、含水量、根系伸长、
叶绿素含量及蛋白表达等的关系。结果显示:随着铜处理浓度的增大,海州香薷根茎叶中的
铜含量均显著增加。10 μmol/L 铜对海州香薷的生长起显著促进作用,高浓度的铜处理造
成对其生长的抑制。100 μmol/L 铜处理下植株的总生物量达到最大,但根伸长和叶绿素含
量均被显著抑制,此时植物根和叶中诱导出一些低分子量的蛋白,可能与海州香薷耐铜性有
关。
关键词:植物学;铜处理;海州香薷;铜积累;铜耐性
中图分类号:
Study on Relations of Copper Accumulation, Growth
Characters and Copper Tolerance in Elsholtzia
haichowensis
Zhang Hongxiao1, Song Yufeng2, Zhang Fenqin3
(1. College of Agriculture, Henan University of Science and Technology,
HeNan LuoYang 471000;
2. College of Life Science, Nanjing Agriculture University, NanJing 210095;
3. Dpartment of Agriculture and biology thechnology,Hexi University, GanSu ZhangYe 734000)
Abstract: Under hydroponics, the relations of copper (Cu) accumulation, growth characters and
proteins expression were investigated in roots, stems and leaves of Elsholtzia haichowensis after
the different concentration of CuSO4 treatment 7 day. Our results showed that Cu treatment
significantly increased Cu accumulation in E. haichowensis, 10 μmol/L Cu treatment significantly
accelerated the growth of E. haichowensis, but the more concentration Cu treatment decreased the
growth of E. haichowensis. The total biomass arrived at the most under 100 μmol/L Cu treatment,
at the same time, this treatment inhibited root elongations and decreased chlorophyll contents in E.
haichowensis, therefore, some low molecular mass proteins in roots and leaves of E. haichowensis
was induced in this Cu treatment, which may be related with the copper tolerance of E.
haichowensis.
Keywords: botany; Cu treatment; Elsholtzia haichowensis; Cu accumulation; Cu tolerance
0 引言
海州香薷(Elsholtzia haichowensis Sun),又名铜草,是广泛分布于铜矿区和铜污染区
的铜指示植物,目前在重金属污染治理中的作用也日益突出。研究发现生长在铜矿区的海州
香薷根部的铜积累量达到