文档介绍：复方黄芪金银花颗粒的质量标准研究
翟少钦曹国文李成君
重庆市畜牧科学院兽医研究所,重庆402460
摘要:目的:建立控制复方黄芪金银花颗粒的质量标准。方法:采用薄层层析对颗粒中女贞子、金银花、黄芪、枸杞子进行了薄层鉴别;采用双光束紫外-可见光分光度法测定了颗粒中绿原酸的含量。结果:薄层鉴别方法专属性强,—µg /ml内具有良好的线性关系,标准曲线回归方程为:A=×C-(r=,n=5);样品在24h内稳定,RSD=%;%,RSD=%;该方法精密度高,RSD=%。结论:本质量标准可有效地控制复方黄芪金银花颗粒的质量。
关键词:黄芪金银花颗粒;薄层色谱法;紫外分光光度法;绿原酸;
Study the standard of pound granules
Zhai shaoqin Cao guowen Li chengjun
Chongqing Academy of Animal Science, Chongqing 402460, China
Abstract: Objective: Establish control standards of pound : Using the Thin layer chromatography to distinguish Astragalus mogholicus, Lonicera japonica, Ligustri lucidi Fructus, Chinese wolfberry fruit, etc. in the particles. Dual-beam UV - visible spectrophotometry in the determination of the particle content of chlorogenic acid. Results: TLC method is specific. Determination of chlorogenic acid has good linearity within the the -µg / ml .The Standard curve regression equation: A=×C-(r=,n=5). The Sample is stable in the 24h, RSD=%. The average recovery is %, RSD=%. This method was high Precision, RSD=%.Conclusion: The quality standards that can effectively control the quality of pound granules.
Keywords: pound granules ,TLC, Dual-beam UV-visible spectrophotometry, Chlorogenic acid
复方黄芪金银花颗粒是本研究所根据中医理论研制的治疗鸡传染性法氏囊病的中药复方制