文档介绍：Biomedical applications of ic nanoparticles : from cell imaging to tissue engineering
Claire Wilhelm
Laboratoire Matière et plexes, UMR 7057, CNRS and Université Paris Diderot, France. claire.******@univ-paris-
Abstract
Recent advances in cell therapy and tissue engineering opened new windows for regenerative medicine, but still necessitate innovative techniques to create and image functional tissues. One promising approach is to associate ic nanoparticles with cells in order to supply them with sufficient ization to be detectable by MRI [1] or manipulated by ic forces [2], while maintaining cell viability and functionalities. A few years ago, we proposed the use of anionic iron oxide nanoparticles as efficient agents for cell internalisation without impacting cell functions [1]. Recently we examined the influence of the amount of internalized iron and the state of nanoparticle aggregation on the capacity for mesenchymal stem cell differentiation and MRI single cell tracking [3]. We then demonstrated that high resolution ic Resonance Imaging (MRI) bining cellular-scale resolution with the ability to detect two cell types simultaneously at any tissue depth [4]. In parallel, we adressed the challenge to create a functional tissue from stem cells in vitro. The aim was to confine stem cells in three dimensions at the millimetric scale by using home-designed miniaturi