文档介绍：PCR-DGGE用于油脂废水处理系统中
酵母菌群落结构解析
吕文洲* 刘英朱建林
(宁波大学建筑工程与环境学院环境工程系宁波 315211)
摘要: 本研究建立了适合于PCR-DGGE分析的环境样品中酵母菌基因组DNA的快速提取方法并利用PCR-DGGE对酵母菌处理油脂废水系统中的酵母菌群落结构进行了解析。结果表明, 并非所有可以降解含油废水中污染物的菌株都可以稳定存在于系统中; 系统对于菌株的选择开始于菌株的扩大培养阶段; 系统从连续运行开始到稳定的过程中, G1、O2或W1成为优势菌株并稳定存在于系统中。
关键词: 酵母菌, DNA提取, PCR-DGGE, 群落结构
Analysis of munity Structure in a Salad Oil-containing Wastewater Treatment System by PCR-DGGE
LÜ Wen-Zhou* LIU Ying ZHU Jian-Lin
(Department of Environmental Engineering, College of Architectural Civil Engineering and Environment,
Ningbo University, Ningbo 315211)
Abstract: A rapid extraction of yeast genomic DNA suitable for PCR-DGGE analysis was established and the munity structure in a salad oil-containing wastewater treatment system was investigated. The results show, not all yeast strains capable of degrading pollutants in wastewater can be planted by system; the selection of system to yeast strains starts from the period of yeast biomass cultivation; from beginning to stable status of continuous wastewater treatment, G1, O2 or W1 are the dominant yeast strains and survive in selection of system.
Keywords: Yeast, DNA extraction, PCR-DGGE, Community structure
Muyzer[1] 于1993年首次将变性梯度凝胶电泳(DGGE)技术引入微生物生态学研究, 并用以确定自然环境中微生物群体的多样性。随后, 该技术被逐渐应用到了废水生物处理过程中对总细菌或功能细菌的微生态结构进行解析[2-7]。近年来, 国内也相继利用此技术对活性污泥系统[8,9]、特种反应
器[10-13]、受重金属污染土壤[14]以及堆肥[15]中的微生物群落结构进行分析, 并快捷、准确地揭示了系统中细菌群落结构的变化及其对系统