文档介绍：312 食品与药品 Food and Drug 2010年第12卷第09期
超临界CO2提取五味子木脂素的工艺研究
戴军1,徐佐旗2,赵婷2,闫舒2,仰榴青2*
(1. 江苏中兴药业有限公司,江苏镇江 212009;2. 江苏大学药学院,江苏镇江 212013)
摘要:目的研究超临界CO2流体提取(SFE-CO2)北、南五味子木脂素的工艺。方法以五味子总木脂素提取率为考察指
标,以提取压力、提取温度、CO2流量等3项因素设计3个水平进行正交试验设计,确定最佳工艺。用薄层扫描色谱法测定
提取物中4种主要木脂素(五味子甲素、五味子乙素、五味子酯甲和五味子醇甲)的含量,并与传统的乙醇提取法比较。
结果超临界CO2流体提取五味子木脂素的优选工艺为:提取压力25 MPa,提取温度35 ℃,CO2流量2 L/min。北、南五
%、 %, %、 %,北五味子提取物中五味子乙素
%。结论 SFE-CO2法提取效率高、选择性好、操作简便,明显优于传统的乙醇提取法,可用于提取五味子木脂
素。
关键词:北五味子;南五味子;超临界CO2提取;正交试验;木脂素;五味子乙素
中图分类号:; 文献标识码:A 文章编号:1672-979X(2010)09-0312-04
Study on Extraction Process of Lignans from Fruits of Schisandra chinensis by Supercritical
CO2 Fluid
DAI Jun1, XU Zuo-qi2, ZHAO Ting2, YAN Shu2, YANG Liu-qing2
( Zhongxing Pharmaceutical Co., Ltd., Zhenjiang 212009, China; University, Zhenjiang 212013,
China)
Abstract: Objective To study the extraction process of lignans in Schisandra chinensis (Turcz.) Baill. and S.
sphenanthera Wils. by supercritical CO2 fluid (SFE-CO2). Methods The orthogonal experimental design was
used to optimize the SFE-CO2 conditions including extraction pressure, extraction temperature and flow rate of CO2,
which was guided by the extraction rate of total lignans. The contents of four lignans in the extracts of Schisandra
chinensis and S. sphenanthera were determined s