文档介绍：目录
水葫芦内生细菌的分离与鉴定 1
桑树细菌性枯萎病新病原菌GFP基因转导 10
茄青枯雷尔氏菌Ralstonia solanacearum和桑细菌性枯萎病菌土壤拮抗菌的筛选 13
基于BOX-PCR的GFP标记的青枯雷尔氏菌的多态性分析 17
GFP标记的青枯雷尔氏菌HR反应的检测 19
野生蕉内生细菌拮抗特性的研究 22
复合凝聚法制备Bt杀虫蛋白明胶-阿拉伯胶微胶囊 32
生防菌JK-2发酵液经80%乙醇处理后对香蕉枯萎病菌的抑制作用 35
生防菌对青枯雷尔氏菌致弱后的分子检测 41
Perl及其编程环境的下载、安装、配置、升级 46
(本期责任编辑:蓝江林,刘波)
水葫芦内生细菌的分离与鉴定收稿日期:
基金项目: 福建省发改委重大项目(闽发高技[2005]1061号)。省财政专项-福建省农业科学院科技创新团队建设基金(STIF-Y03)。
作者简介:蓝江林(1972-),副研究员,研究方向:生物技术与生物防治。电子邮件:lanjianglin2002@。
通讯作者:刘波,博士,研究员。研究方向:生物技术与生物防治。电子邮件:fzliubo@
蓝江林,朱育菁,苏明星,葛慈斌,刘芸,刘波
(福建省农业科学院农业生物资源研究所,福建福州 350003)
摘要:采用20种不同培养基分离水葫芦内生细菌,利用细菌脂肪酸鉴定技术对水葫芦的内生细菌进行鉴定,研究水葫芦内生细菌的种类及群落结构。共得到32个属的56株内生细菌,种类最多的是微杆菌属(Microbacterium),共有9种细菌。其次为假单胞菌属(Pseudomonas),共有7种细菌。芽孢杆菌属(Bacillus)有5种细菌。其余23个属均有1种。利用优势度指数分析各培养基分离到内生细菌的多样性,,分离得到的内生菌种类最多均为7种;其次是16号黄豆芽汁培养基(~)和20号醋酸菌培养基,,分离获得内生细菌均为5种。
关键词:水葫芦,内生细菌
Isolation and identification of the endophyte bacteria from Eichhornia crassipe (Mart.) Solms.
LAN Jiang-lin,ZHU Yu-jing,SU Ming-xing, GE Ci-bin,LIU Yun,WANG Qiu-hong,Liu Bo**
(Agricultural Bioresource Research Institute,Fujian Academy of Agricultural Sciences, Fuzhou 350003)
Abstract:Endophyte bacteria were isolated from the plant tissues of Eichhornia crassipe (Mart.) Solms. by using 20 types of cultural media. The collected 56 bacterial isolates were identified with bacterial fatty-acid identification system to study the population structure of endophyte bacteria inside E. crassipe. The endophyte bacteria were classified into 32 genera, while most belonged to Microbacterium with 9 isolates, Pseudomonas with 7 isolates and Bacillus with 5 isolatesa as well as the other 23 genus had only 1 isolate diversity of endiophyte bacterica on culture medium using dominance index,result show dominance index of Rhizobiumv culture medium and NA culture medium is ,had 7 isolate is soybean sprouts juice culture medium and Acetobacter Aceti culture medium,dominance ind