文档介绍：3TSR对胆囊癌的抑制作用及初步机制
金洲祥,朱少俊, 张雪峰, 章小平, 王飞海
[摘要] 目的研究血管形成抑制剂3TSR对裸鼠皮下胆囊癌种植瘤的抑制作用及其机制。方法建立裸鼠皮下胆囊癌种植瘤模型,进行随机干预实验,实验分为对照组、3TSR组、5-氟尿嘧啶(5-FU)组和3TSR+5-FU组,每组6只。检测荷瘤体积、胆囊癌细胞增殖指数(PCNA)、凋亡率、微血管密度(MVD)、肿瘤血管内皮细胞凋亡率及血管内皮生成因子(VEGF)的表达。结果(1)3TSR组、5-FU组和3TSR+5-FU组肿瘤体积较对照组显著减小,%,%%。对照组、3TSR组、5-FU组和3TSR+5-FU组胆囊癌细胞增殖指数分别为(±)% ,(±)%, (±)%和(±)%,其中3TSR组增殖指数与对照组比较无显著差异,但5-FU组和3TSR+5-FU组细胞增殖指数显著小于对照组和3TSR组(P<)。4组胆囊癌细胞凋亡率分别为(±)%,(±)%, (±)%和(±)%,其中3TSR组胆囊癌细胞凋亡率与对照组比较无显著差异(P<),但5-FU组和3TSR+5-FU组细胞凋亡率显著大于对照组和3TSR组(P<)。4组微血管密度分别为(±)%,(±)%、(±)%和(±)%,其中含3TSR的两组显著低于对照组和5-Fu组(P<)。4组内皮细胞凋亡率分别为(±) %,(±)%, (±)%, (±)%,其中含3TSR组显著高于对照组(P<)。4组VEGF表达率分别为(±)% ,(±) %,(±)%和(±)%,其中含3TSR的两组显著低于对照组和5-Fu组(P<)。结论 3TSR显著减少肿瘤体积,抑制微血管生成,其机制可能与诱导内皮细胞凋亡和抑制VEGF有关;3TSR与化疗药物5-FU合用对胆囊癌的治疗有协同作用。本研究为3TSR的临床前期应用提供了一定实验基础。
关键词:胆囊癌;血管形成;3TSR;凋亡;血管内皮生成因子
资金项目:浙江省教育厅自然科学基金资助(20051188)
作者单位:325027 温州,温州医学院附属第二医院普通外科(金洲祥,朱少俊,徐鲁白,王继生);美国哈佛医学院Beth Isreal Deaconess医学中心(张雪峰,章小平)
通信作者:金洲祥,E-mail:jzx0847@
The inhibitory effects of 3TSR on gallbladder cancer and its rudimentary mechanism
Zhouxiang Jin*,Shaojun Zhu ,Xuefeng Zhang,et al.*Department of Surgery, The second affliated hospital of Wenzhou medical college, Wenzhou,Zhejiang , 325027, China.
Corresponding author: Zhouxiang Jin
[ABSTRACT] Objective To investigate the anti-tumor efficacy of angiogenic inhibitor 3TSR on gallbladdercancer cells and explore its rudimentary mechanism. Methods Human gallbladder carcinoma cell of the line GBC-SD were inoculated subcutaneously with BALB/c mice ,and then the mice were divided into four groups:control group,3TSR group,5-FU group and 3TSR plus 5-FU group with 6 mice each group. The tumor volume,cancer cell proliferation, apoptosis rate , endothelial cell apoptosis rate and expression of vascular endothelial growth factor(VEGF) were detected, respectively. Resu