文档介绍：论著
近红外线激光拉曼光谱用于胃癌诊断的研究
毛华, 金少琴
作者单位:广东省广州市南方医科大学珠江医院消化科 510280
通讯作者:毛华, Tel: **********, E-mail: Huam@,
【摘要】目的本研究采用近红外线激光拉曼光谱技术研究胃癌组织、胃癌前病变组织及正常胃粘膜组织的拉曼光谱的差异变化,建立一种胃癌早期诊断的方法。方法收集病理学证实的12例胃癌组织、12例胃癌前病变组织及21例正常胃粘膜组织,用近红外线激光拉曼光谱仪进行检测胃病变组织拉曼峰强度比值,所有标本来自45例受试者均为接受胃切除或是接受胃镜下对胃部可疑病灶进行活检的患者。结果三类不同病理类型的胃组织标本的典型拉曼峰位于853cm-1,1004cm-1,1337cm-1,144 5cm-1,1655cm-1,1310-1350cm-1, 1640-1657cm-1波长处,在1337cm-1,1445cm-1和1655cm-1波长处,胃癌组的拉曼峰强度值高于健康对照组,而胃癌前病变组介于两者之间(p<)。拉曼峰强度比值I1003/I1337,I1003/ I1445,I1003 /I1655,I1156/I1655,健康对照组显著高于胃癌组及胃癌前病变组 p<(差异有统计学意义)。采用PCA-LDA %,%,%,%。结论近红外线激光拉曼光谱分析技术联合拉曼峰强度比值具有在分子水平诊断胃癌的价值。
【关键词】近红外线拉曼光谱,胃癌,胃癌前病变,早期诊断。
Near-infrared Raman spectroscopy for gastric cancer diagnosis
Mao Hua,Jin Shao-qin
Dept. of Gastroenterology,ZhujiangHosiptal,Southern Medical University, Guangzhou, China 510282
Co-responding author:Mao Hua, Tel:**********, E-mail: Huam@,
【Abstract】 Objective The purpose of this study was to apply Near-infrared Raman spectroscopy for differentiating gastric cancer and gastric precancerous lesions and normal gastric mucosa
,establishing a method for an early diagnosis of gastric A rapid NIR Raman system was used for tissue measurements, 45 gastric tissue samples from 45 patients who underwent endoscopy or gastrectomy operation were used (21 normal tissue specim