文档介绍：脑干热休克蛋白70的表达与实验性脑损伤猝死的相关性
作者:赵连旭1;徐小虎2;刘超3;潘速跃4;祝家镇4;张成4 (1第一军医大学珠江医院神经内科, 广东广州510282;2汕头大学医学院,广东汕头515030; 3广州市刑警支队,广东广州510030;4第一军医大学南方医院神经内科,广东广州510515)
摘要:目的  了解脑损伤后热休克蛋白70 (Heat-shock protein 70, HSP70) 蛋白质及mRNA在脑干局部的改变,及其对维持脑干生命中枢功能的作用。方法  用头颅打击器制成大鼠脑损伤猝死模型,免疫组化法分别测定损伤组损伤前和损伤后1、3、6、12 h及损伤猝死组脑干HSP70的数量和分布变化;逆转录多聚酶链反应(RT-PCR)检测各组相应部位HSP70 mRNA水平。结果  在脑损伤15 min后脑干HSP70 mRNA水平显著升高, HSP70蛋白质需3~6 h才大量表达;损伤后猝死的鼠脑中仅见HSP70 mRNA升高,HSP70蛋白质与对照组无差异。结论  脑损伤后HSP70合成迅速增加,清除损伤因素,维持自稳状态;当损伤严重时,应激蛋白质的合成障碍,抗损伤的保护机制不能发挥,脑干生命中枢的功能紊乱,生命丧失。因此, HSP70 mRNA-蛋白质分离可作为神经细胞致死性损伤的标志。
关键词: 热休克蛋白类; 脑损伤; 免疫组织化学; 多聚酶链式反应
中图分类号: Q51;   文献标识码: A  文章编号: 1000-2588 (2001) 01-0005-03
Correlation between expression of heat-shock protein 70 in the brain stem and sudden death after traumatic brain injury:an experimental study
ZHAO Lian-xu1;XU Xiao-hu2;LIU Chao3;PAN Su-yue4;ZHU Jia-zhen4;ZHANG Cheng
4
(1Department of Neurology, Zhujiang Hospital, First Military Medical University, Guangzhou 510282,China; 2Shantou University Medical College, Shantou 515030,China; 3Department of Criminal Police,Guangzhou Public Security Bureau, Guangzhou 510030,China; 4Department of Neurology, Nanfang Hospital, First Military Medical University,Guangzhou 510515, China )
Abstract: Objective  To investigate the patterns of heat-shock protein 70 (HSP70) biosynthesis following traumatic brain injury and observe its effect on the function of the brain stem. Methods Animal models of traumatic brain injury were established in rats using self-designed devices, and the expression of HSP70 in the brain stem 1,3,6,12 h after the injury in injury group and immediately after death in sudden death group respectively,was determined immunohistochemically. HSP70 mRNA was measured by RT-PCR 15,30 min and 1,3,6 h after injury and following sudden death respectively.  Results In the injury group, the level of HSP70 mRNA in the brain stem was evidently elevated as early as 15 min following  impact-induced brain injury,while prof