文档介绍：细胞表面唾液酸及其连接方式对乳腺癌细胞黏附的影响
作者:王晓玉1;林绍强2;李君武2;Wolfgang Kemmner3;丁彦青4 (暨南大学1第一医院妇产科,2医学院微生物与免疫学教研室,广东  广州  510630;3Max-Delbrueck Center for Molecular Medicine,Berlin 13125, Germany;4南方医科大学肿瘤学研究所,广东  广州  510515)
摘要:目的  研究肿瘤细胞总唾液酸及α2,6-连接唾液酸对乳腺癌细胞MDA-MB435黏附力的影响。方法  乳腺癌细胞MDA-MB-435转染顺义或反义α2,6唾液酸转移酶(ST6Gal-I) cDNA,以黑接骨木凝集素SNA筛选高、低表达α2,6唾液酸细胞克隆,检测各转染细胞的同源凝集作用,并比较唾液酸酶处理前后肿瘤细胞对胶原IV黏附力的变化。结果顺义转染克隆细胞-细胞之间的同源黏附能力下降,与胶原IV的黏附增强;反义转染克隆细胞-细胞之间同源黏附能力增强,而与胶原IV的黏附降低。唾液酸酶处理后肿瘤细胞与胶原IV的黏附力下降至未处理前的31%~57%。结论  乳腺癌MDA-MB435细胞表面唾液酸及α-2,6唾液酸化对肿瘤细胞的黏附有重要影响。
关键词:乳腺肿瘤;唾液酸转移酶;细胞外基质;细胞黏附;肿瘤转移
中图分类号:R737  文献标识码:A  文章编号:1673-4254(2006)06-0742-05
Effect of cell surface sialic acid and their linkages on adhesion of mammary carcinoma cells
WANG Xiao-yu1; LIN Shao-qiang2; LI Jun-wu2; Kemmner Wolfgang3; DING Yan-qing4
Department of Obstetrics and Gynecology, First Affiliated Hospital of Ji’nan University
1, Department of Microbiology and Immunology of Medical College2, Jinan University, Guangzhou 510630, China; 3Max-Delbrueck Center for Molecular Medicine,Berlin 13125, Germany; 4Institute of Oncology, Southern Medical University, Guangzhou 510515, China
Abstract: Objective  To investigate the effect of cell surface sialic acid and its linkage on the cell-cell and cell-matrix adhesion of mammary carcinoma cells MD-MB-435. Methods MD-MB-435 cells were sense-transfected with ST6Gal I cDNA or antisense-transfected with part of the ST6Gal I sequence inserted in pcDNA vector, with mock transfection with vector as the control. The cell surface α2, 6-linked sialylation was determined by fluorescence-activated cell sorting (FACS) using lectin SNA (Sambucus nigra agglutinin specific to α2, 6-linked sialic acid on N-linked glycoprotein). A significantly increased α2, 6-sialylation subclone in sense-tranfectants and a decreased α2, 6-sialylation subclone in antisense-transfectants were selected for further examination of cell-cell and cell-matrix (collagen IV) adhesion. The transfectants were al