文档介绍：全骨髓法体外培养分离大鼠骨髓间充质干细胞及PKH26标记**★
叶小凤,何援利,王雪峰,付霞霏
Bone marrow mesenchymal stem cells isolated from rats by whole bone marrow adherent culture in vitro and PKH26 labeling
Ye Xiao-feng, He Yuan-Li, Wang Xue-feng, Fu Xia-fei
Department of Obstetrics and Gynecology, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, Guangdong Province, China
Ye Xiao-feng★, Studying for master’s degree, Department of Obstetrics and Gynecology, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, Guangdong Province, China
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Correspondence to: He Yuan-li, Chief physician, Professor, Department of Obstetrics and Gynecology, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, Guangdong Province, China
Supported by: the National Natural Science Foundation of China, No. 81041101*; the Natural Science Foundation of Guangdong Province, No. 10451051501004 704*
Received: 2010-12-03
Accepted: 2011-01-17
Abstract
BACKGROUND: In vitro culturing, amplification and labeling are important links to harvest labeled rat bone marrow mesenchymal stem cells (BMSCs) that are satisfactory to animal experiment.
OBJECTIVE: To explore a convenient and practical method for separating and labeling BMSCs using whole bone marrow adherent culture, and labeling with PKH26 in vitro.
METHODS: BMSCs were isolated and cultivated from the bone marrow of rats by whole bone marr